Active • Host: E. coli • AA: 2-106 (full length) • Tag: N-terminal His-TEV • MW: 13.9 kDa
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Glutaredoxin 1 (human, recombinant)

Item No. 31037

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Glrx1
  • Grx1
  • Thioltransferase 1
  • TTF1
Purity
≥95% estimated by SDS-PAGE
Source
Active human recombinant N-terminal His-TEV tagged Grx1 expressed in E. coli
Amino Acids
2-106
MW
13.9 kDa
Formulation (Request formulation change)
50 mM Tris-HCl, pH 7.8, with 150 mM sodium chloride, 10% glycerol, 1 mM EDTA, and 0.1 mM DTT
UniProt Accession №
P35754
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $423

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    Cayman Chemical
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    Product Description

    Glutaredoxin 1 (Grx1) is a thiol-disulfide oxidoreductase and member of the thioredoxin family encoded by GLRX1 with a role in the maintenance of cellular thiol redox homeostasis.1 It is ubiquitously expressed and localizes to the cytosol, nucleus, and mitochondrial intermembrane space. Grx1 is a dithiol Grx that contains two active site cysteine residues, which catalyze the deglutathionylation of protein disulfides and glutathionylated proteins to regulate redox signal transduction and repair oxidized proteins.2,3,4 Knockdown or overexpression of GLRX1 enhances or reduces, respectively, oxidative stress-induced apoptosis in HEK293T cells.5 Glrx1-/- mice have increased bronchoalveolar lavage fluid (BALF) macrophage infiltration and lung glutathionylated protein levels induced by cigarette smoke compared with wild-type mice.6 Grx1 levels are decreased in postmortem brain from patients with Parkinson's disease.4 Cayman's Glutaredoxin 1 (human, recombinant) can be used for enzyme activity assays.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hanschmann, E.-M., Godoy, J.R., Berndt, C., et al. Thioredoxins, glutaredoxins, and peroxiredoxins—molecular mechanisms and health significance: From cofactors to antioxidants to redox signaling. Antioxid. Redox Signal. 19(13), 1539-1605 (2013).

    2. Matsui, R., Ferran, B., Oh, A., et al. Redox regulation via glutaredoxin-1 and protein S-glutathionylation. Antioxid. Redox Signal. 32(10), 677-700 (2020).

    3. Lillig, C.H., Berndt, C., Vergnolle, O., et al. Characterization of human glutaredoxin 2 as iron-sulfur protein: A possible role as redox sensor. Proc. Natl. Acad. Sci. USA 102(23), 8168-8173 (2005).

    4. Miller, O.G. Critical roles of glutaredoxin in brain cells-implications for Parkinson’s disease. Antioxid. Redox Signal. 30(10), 1352-1368 (2018).

    5. Yang, F., Yi, M., Liu, Y., et al. Glutaredoxin-1 silencing Induces cell senescence via p53/p21/p16 signaling axis. J. Proteome Res. 17(3), 1091-1100 (2018).

    6. Kuipers, I., Bracke, K.R., Brusselle, G.G., et al. Altered cigarette smoke-induced lung inflammation due to ablation of Grx1. PLoS One 7(6), e38984 (2012).