A lanthanide-based fluorophore for TR-FRET applications
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Europium W1024 ITC

Item No. 31103

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
[[N,N′-[[4-[(4-isothiocyanatophenyl)ethynyl]-2,6-pyridinediyl-κN]bis(methylene)]bis[N-[(carboxy-κO)methyl]glycinato-κN,κO]](4-)]-europate(1-), monosodium salt
CAS Number
1204652-02-7
Synonyms
  • Eu-W1024 ITC
  • Europium W1024 Isothiocyanate
Molecular Formula
C24H18EuN4O8S • Na
Formula Weight
697.4
Emission
613 nm
Excitation
340 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Water: Soluble
SMILES
O=C1[O-][Eu+3]2([N](C3)(C1)C4)([O-]C3=O)([O-]C5=O)([O-]C6=O)[N](C6)(C5)CC7=[N]2C4=CC(C#CC8=CC=C(N=C=S)C=C8)=C7.[Na+]
InChi Code
InChI=1S/C24H22N4O8S.Eu.Na/c29-21(30)11-27(12-22(31)32)9-19-7-17(2-1-16-3-5-18(6-4-16)25-15-37)8-20(26-19)10-28(13-23(33)34)14-24(35)36;;/h3-8H,9-14H2,(H,29,30)(H,31,32)(H,33,34)(H,35,36);;/q;+3;+1/p-4
InChi Key
HBTYYFSVTSFIGH-UHFFFAOYSA-J
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    2 mg

    $9,537

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    Product Description

    Europium W1024 isothiocyanate (ITC) is a lanthanide-based fluorophore with a long emission lifetime and large Stokes shift.1,2 It displays excitation/emission maxima of 340/613 nm, respectively.1 Europium W1024 ITC contains a reactive isothiocyanate handle that reacts with primary amino groups at an alkaline pH. It is stable in the presence of high temperature, high EDTA concentration, or low pH but not in the presence of manganese. Europium W1024 ITC has commonly been conjugated to various molecules and used as a donor fluorophore in TR-FRET where its long emission lifetime provides a longer window of measurement for acceptor fluorescence and reduces the interference of autofluorescence.3,4,5 For Cayman's recommended protein labeling protocol please see the product information sheet.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Mathis, G., and Bazin, H. Stable luminescent chelates and macrocyclic compound. Lanthanide luminescence: Photophysical, analytical and biological aspects 7, 47-88 (2010).

    2. Mathis, G. Probing molecular interactions with homogeneous techniques based on rare earth cryptates and fluorescence energy transfer. Clin. Chem. 41(9), 1391-1397 (1995).

    3. Moshinsky, D.J., Ruslim, L., Blake, R.A., et al. A widely applicable, high-throughput TR-FRET assay for the measurement of kinase autophosphorylation: VEGFR-2 as a prototype. J. Biomol. Screen. 8(4), 447-452 (2003).

    4. Scheepstra, M., Leysen, S., van Almen, G.C., et al. Identification of an allosteric binding site for RORγt inhibition. Nat. Commun. 6, 8833 (2015).

    5. Poore, D.D., Hofmann, G., Wolfe, L.A., 3rd., et al. Development of a high-throughput Cul3-Keap1 time-resolved fluorescence resonance energy transfer (TR-FRET) assay for identifying Nrf2 activators. SLAS Discov. 24(2), 175-189 (2019).