A fluorescent calcium indicator
Technical Support & Resources

Information provided in the product description is from published literature. Due to the nature of scientific experimentation, your results (e.g., selectivity and effective concentrations) or specific application for this product may differ. If you have questions about how this product fits your application, please contact our technical support staff.

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Fluo-4 AM

Item No. 31143

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-glycine (acetyloxy)methyl ester
CAS Number
273221-67-3
Synonyms
  • Fluo-4 Acetoxymethyl ester
Molecular Formula
C51H50F2N2O23
Formula Weight
1096.9
Purity
≥95%
Emission
520 nm
Excitation
490 nm
Formulation (Request formulation change)
A solid
Solubility(Learn about Variance in Solubility)
Methanol: soluble
SMILES
FC1=C(OCOC(C)=O)C=C(O2)C(C(C3=CC(OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4)=C(C=C3)N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(C2=CC5=O)C=C5F)=C1
InChi Code
InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
InChi Key
QOMNQGZXFYNBNG-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $468

    Add
    Product Description

    Fluo-4 acetoxymethyl ester (Fluo-4 AM) is a cell-permeable fluorescent calcium indicator.1 It is cleaved by intracellular esterases to release Fluo-4, which binds to calcium with a Kd value of 345 nM and displays excitation/emission maxima of 490/520 nm, respectively. Fluo-4 AM has been used to measure intracellular calcium levels in live cells by fluorescent microscopy or flow cytometry.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gee, K.R., Brown, K.A., Chen, W.N., et al. Chemical and physiological characterization of fluo-4 Ca2+ - indicator dyes. Cell Calcium 27(2), 97-106 (2000).

    2. Vines, A., McBean, G.J., and Blanco-Fernández, A. A flow-cytometric method for continuous measurement of intracellular Ca2+ concentration. Cytometry A. 77(11), 1091-1097 (2010).