For immunochemical detection of the SARS-CoV surface glycoprotein
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SARS-CoV Spike Glycoprotein S1 Subunit Monoclonal Antibody (Clone 02)

Item No. 31431

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • SARS-CoV Surface Glycoprotein S1 Subunit
  • Severe Acute Respiratory Syndrome Coronavirus Spike Glycoprotein S1 Subunit
Immunogen
Recombinant SARS-CoV surface glycoprotein S1 subunit
Clone Designation
02
Formulation (Request formulation change)
50 or 100 µl of protein A-purified monoclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS
Host
Mouse
Isotype
IgG1
Applications
ELISA
Species Reactivity
(+) SARS-CoV(-) SARS-CoV-2(-) MERS-CoV(-) HCoV-HKU1 (Isolate N1)(-) HCoV-HKU1 (Isolate N5)(-) HCoV-229E(-) HCoV-OC43(-) HCoV-NL63
UniProt Accession №
P59594
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µl

    $164

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    100 µl

    $279

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    Cayman Chemical
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    Product Description

    Severe acute respiratory syndrome coronavirus (SARS-CoV) surface glycoprotein, also known as the spike glycoprotein, is encoded by the S gene in SARS-CoV RNA.1 SARS-CoV is a member of the Betacoronavirus genus of viruses and has an approximately 79% sequence identity with SARS-CoV-2, the causative agent of COVID-19.2,3 The spike protein of SARS-CoV and the related viruses SARS-CoV-2 and Middle East respiratory syndrome coronavirus (MERS-CoV) is a transmembrane glycoprotein that assembles into homotrimers on the virus surface and is comprised of an N-terminal S1 subunit, which contains the receptor binding domain (RBD), and a C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes.4,5,6 The 193-amino acid RBD of the SARS-CoV spike protein is a target for neutralizing antibodies.5,7 The SARS-CoV RBD, which spans amino acid residues 318 to 510, is 73% identical to that of SARS-CoV-2 and can bind to human angiotensin-converting enzyme 2 (ACE2), which is the host cell surface receptor for both SARS-CoV and SARS-CoV-2.4,5,6,7 SARS-CoV is the causative agent of SARS, a primarily respiratory illness characterized by fever, cough, shortness of breath, and an approximately 10% fatality rate.3 Cayman's SARS-CoV Surface Glycoprotein S1 Subunit Monoclonal Antibody (Clone 02) can be used for ELISA. The antibody recognizes the S1 subunit to detect the SARS-CoV surface glycoprotein.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kandeel, M., Ibrahim, A., Fayez, M., et al. From SARS and MERS CoVs to SARS-CoV-2: Moving toward more biased codon usage in viral structural and nonstructural genes. J. Med. Virol. 92(6), 660-666 (2020).

    2. Lu, R., Zhao, X., Li, J., et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: Implications for virus origins and receptor binding. Lancet 395(10224), 565-574 (2020).

    3. Meo, S.A., Alhowikan, A.M., Al-Khlaiwi, T., et al. Novel coronavirus 2019-nCoV: Prevalence, biological and clinical characteristics comparison with SARS-CoV and MERS-CoV. Eur. Rev. Med. Pharmacol. Sci. 24(4), 2012-2019 (2020).

    4. Liu, Z., Xiao, X., Wei, X., et al. Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS-CoV-2. J. Med. Virol. 92(6), 595-601 (2020).

    5. He, Y., Zhou, Y., Liu, S., et al. Receptor-binding domain of SARS-CoV spike protein induces highly potent neutralizing antibodies: Implication for developing subunit vaccine. Biochem. Biophys. Res. Commun. 324(2), 773-781 (2004).

    6. Walls, A.C., Park, Y.-J., Tortorici, M.A., et al. Structure, function, and antigenicity of the SARS-CoV-2 spike glycoprotein. Cell 181(2), 281-292 (2020).

    7. Tian, X., Li, C., Huang, A., et al. Potent binding of 2019 novel coronavirus spike protein by a SARS coronavirus-specific human monoclonal antibody. Emerg. Microbes Infect. 9(1), 382-385 (2020).