A colorimetric reagent that can be used in the detection of iron
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3,3′-[3-(2-Pyridinyl)-1,2,4-triazine-5,6-diyl]bis[benzenesulfonic acid] (sodium salt)

Item No. 31465

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
3,3′-[3-(2-pyridinyl)-1,2,4-triazine-5,6-diyl]bis-benzenesulfonic acid, monosodium salt
CAS Number
1353005-64-7
Molecular Formula
C20H14N4O6S2 • Na
Formula Weight
493.5
Purity
≥98% (mixture of isomers)
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
PBS (pH 7.2): 1 mg/ml
λmax
287 nm
SMILES
O=S(O)(C1=CC(C2=C(C3=CC(S(=O)(O)=O)=CC=C3)N=C(C4=CC=CC=N4)N=N2)=CC=C1)=O.[Na]
InChi Code
InChI=1S/C20H14N4O6S2.Na/c25-31(26,27)15-7-3-5-13(11-15)18-19(14-6-4-8-16(12-14)32(28,29)30)23-24-20(22-18)17-9-1-2-10-21-17;/h1-12H,(H,25,26,27)(H,28,29,30);
InChi Key
WISSPEMMEQKVEH-UHFFFAOYSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 g

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    Product Description

    3,3′-[3-(2-Pyridinyl)-1,2,4-triazine-5,6-diyl]bis[benzenesulfonic acid] is a derivative of the colorimetric iron reagent ferrozine, which has commonly been used as a colorimetric reagent in the detection of iron.1,2 While ferrozine was originally reported to contain sulfonate groups in the 4,4’ positions, some commercial preparations have been found to primarily contain other isomers, including an isomer with a 3,3'-sulfate substitution: 3,3′-[3-(2-pyridinyl)-1,2,4-triazine-5,6-diyl]bis[benzenesulfonic acid].1,3 Compounds containing the pyridyl-triazyl head group, which is found in ferrozine and its isomers, including the 3,3'-sulfonate-containing isomer, form a complex with ferrous iron (Fe2+) that can be quantified as a measure of iron concentration.1,4,5 Ferrozine-iron complexes can be quantified by colorimetric detection at 562 nm. This product primarily contains the 3,3ʹ-sulfonate isomer with 4,4'-, 3,4'-, and 4,3'-sulfonate isomers as secondary or minor components.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Stookey, L.L. Ferrozine-A new spectrophotometric reagent for iron. Anal. Chem. 42(7), 779-781 (1970).

    2. Jeitner, T.M. Optimized ferrozine-based assay for dissolved iron. Anal. Biochem. 454, 36-37 (2014).

    3. Riis-Johannessen, T., Schenk, K., and Severin, K. Turn-off-and-on: Chemosensing ensembles for sensing chloride in water by fluorescence spectroscopy. Inorg. Chem. 49(20), 9546-9553 (2010).

    4. Hirayama, T., and Nagasawa, H. Chemical tools for detecting Fe ions. J. Clin. Biochem. Nutr. 60(1), 39-48 (2017).

    5. Eskelinen, S., Haikonen, M., and Räisänen, S. Ferene-S as the chromogen for serum iron determinations. Scand. J. Clin. Lab. Invest. 43(5), 453-455 (1983).