An aminoxanthene dye
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Sulforhodamine B (sodium salt) (technical grade)

Item No. 31539

Technical Information
Formal Name
3,6-bis(diethylamino)-9-(2,4-disulfophenyl)-xanthylium, inner salt, monosodium salt
CAS Number
3520-42-1
Synonyms
  • Acid Red XB
Molecular Formula
C27H29N2O7S2 • Na
Formula Weight
Purity
≥75%
A crystalline solid
DMF: 10 mg/mlDMSO: 10 mg/mlEthanol: 10 mg/mlPBS (pH 7.2): 10 mg/ml
λmax
553 nm
SMILES
CCN(CC)C1=CC2=[O+]C3=C(C=CC(N(CC)CC)=C3)C(C4=C(S([O-])(=O)=O)C=C(S([O-])(=O)=O)C=C4)=C2C=C1.[Na+]
InChi Code
InChI=1S/C27H30N2O7S2.Na/c1-5-28(6-2)18-9-12-21-24(15-18)36-25-16-19(29(7-3)8-4)10-13-22(25)27(21)23-14-11-20(37(30,31)32)17-26(23)38(33,34)35;/h9-17H,5-8H2,1-4H3,(H-,30,31,32,33,34,35);/q;+1/p-1
InChi Key
SXQCTESRRZBPHJ-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    Product Description

    Sulforhodamine B is an aminoxanthene dye. It binds basic amino acid residues under mild acidic conditions, which can be quantified by colorimetric detection at 510 nM, and is commonly used for cell density quantification in cytotoxicity screening.1,2

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Skehan, P., Storeng, R., Scudiero, D., et alNew colorimetric cytotoxicity assay for anticancer-drug screening. J. Natl. Cancer Inst. 82(13), 1107-1112 (1990).

    2. Vichai, V., and Kirtikara, K. Sulforhodamine B colorimetric assay for cytotoxicity screening. Nat. Protoc. 1(3), 1112-1116 (2006).