A colorimetric plasmin substrate
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N-p-Tosyl-Gly-Pro-Lys-pNA (acetate)

Item No. 31544

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
N-[(4-methylphenyl)sulfonyl]glycyl-L-prolyl-N-(4-nitrophenyl)-L-lysinamide, monoacetate
CAS Number
88793-79-7
Synonyms
  • N-p-Tosyl-Gly-Pro-Lys-p-nitroanilide
Molecular Formula
C26H34N6O7S • C2H4O2
Formula Weight
634.7
Purity
≥98%
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
Ethanol: 50 mg/ml
λmax
226 nm
SMILES
CC(O)=O.O=C(NC1=CC=C(C=C1)[N+]([O-])=O)[C@H](CCCCN)NC([C@H]2N(C(CNS(C3=CC=C(C)C=C3)(=O)=O)=O)CCC2)=O
InChi Code
InChI=1S/C26H34N6O7S.C2H4O2/c1-18-7-13-21(14-8-18)40(38,39)28-17-24(33)31-16-4-6-23(31)26(35)30-22(5-2-3-15-27)25(34)29-19-9-11-20(12-10-19)32(36)37;1-2(3)4/h7-14,22-23,28H,2-6,15-17,27H2,1H3,(H,29,34)(H,30,35);1H3,(H,3,4)/t22-,23-;/m0./s1
InChi Key
KPGSNEYSJPIZCW-SJEIDVEUSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
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    1 mg

    $38

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    5 mg

    $165

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    10 mg

    $291

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    Product Description

    N-p-Tosyl-Gly-Pro-Lys-pNA is a colorimetric substrate for plasmin.1,2,3 Plasmin binds and hydrolyzes N-p-tosyl-Gly-Pro-Lys-pNA to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of plasmin activity.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Jespersen, J., Gram, J., and Astrup, T. The autodigestion of human plasmin follows a bimolecular mode of reaction subject to product inhibition. Thromb. Res. 41(3), 395-404 (1986).

    2. Kim, S.H., and Choi, N.S. Electrophoretic analysis of protease inhibitors in fibrin zymography. Anal. Biochem. 270(1), 179-181 (1999).

    3. Chen, T., and Rael, E.D. Purification of M5, a fibrinolytic proteinase from Crotalus molossus molossus venom that attacks complement. Int. J. Biochem. Cell Biol. 29(5), 789-799 (1997).