A water-soluble and cell-permeable tetrazolium dye
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WST-1

Item No. 31752

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
5-(2,4-disulfophenyl)-2-(4-iodophenyl)-3-(4-nitrophenyl)-2H-tetrazolium, inner salt, monosodium salt
CAS Number
150849-52-8
Synonyms
  • Water-Soluble Tetrazolium 1
Molecular Formula
C19H11IN5O8S2 • Na
Formula Weight
651.3
Formulation (Request formulation change)
A crystalline solid
Solubility(Learn about Variance in Solubility)
DMF: 5 mg/mlDMSO: 10 mg/mlPBS (pH 7.2): 10 mg/ml
SMILES
[O-]S(C(C=C1)=CC(S([O-])(=O)=O)=C1C2=NN(C3=CC=C(I)C=C3)[N+](C4=CC=C([N+]([O-])=O)C=C4)=N2)(=O)=O.[Na+]
InChi Code
InChI=1S/C19H12IN5O8S2.Na/c20-12-1-3-13(4-2-12)23-21-19(22-24(23)14-5-7-15(8-6-14)25(26)27)17-10-9-16(34(28,29)30)11-18(17)35(31,32)33;/h1-11H,(H-,28,29,30,31,32,33);/q;+1/p-1
InChi Key
JUJBNYBVVQSIOU-UHFFFAOYSA-M
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    10 mg

    $90

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    50 mg

    $197

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    100 mg

    $349

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    250 mg

    $762

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    Product Description

    WST-1 is a water-soluble and cell-permeable tetrazolium dye.1,2,3 Upon NADH-dependent enzymatic cleavage by cellular mitochondrial dehydrogenases, formazan is released, which can be quantified by colorimetric detection at 450 nm as a measure of cell viability.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Francoeur, A.M., and Assalian, A. Microcat: A novel cell proliferation and cytotoxicity assay based on WST-1. Biochemica 3, 19-25 (1996).

    2. Ishiyama, M., Shiga, M., Sasamoto, K., et al. A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem. Pharm. Bull. 41(6), 1118-1122 (1993).

    3. Ishiyama, M., Tominaga, H., Shiga, M., et al. A combined assay of cell viability and in vitro cytotoxicity with a highly water-soluble tetrazolium salt, neutral red and crystal violet. Biol. Pharm. Bull. 19(11), 1518-1520 (1996).