An internal standard for the quantification of furosemide
Related Products
Unlabeled Version(s)
17273Furosemide
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Furosemide-d5

Item No. 31789

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Formal Name
4-chloro-2-(((furan-2-yl-d3)methyl-d2)amino)-5-sulfamoylbenzoic acid
CAS Number
1189482-35-6
Synonyms
  • Frusemide-d5
Molecular Formula
C12H6ClD5N2O5S
Formula Weight
335.8
Purity
≥99% deuterated forms (d1-d5)
Formulation
A solid
Solubility(Learn about Variance in Solubility)
Chloroform: Slightly solubleMethanol: Slightly soluble
SMILES
ClC1=C(S(N)(=O)=O)C=C(C(O)=O)C(NC([2H])([2H])C2=C([2H])C([2H])=C([2H])O2)=C1
InChi Code
InChI=1S/C12H11ClN2O5S/c13-9-5-10(15-6-7-2-1-3-20-7)8(12(16)17)4-11(9)21(14,18)19/h1-5,15H,6H2,(H,16,17)(H2,14,18,19)/i1D,2D,3D,6D2
InChi Key
ZZUFCTLCJUWOSV-JZOBIDBQSA-N
Shipping & Storage Information
Storage
-20°C
Shipping
Room temperature in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

Download most recent Certificate of Analysis for this product

    1 mg

    $363

    Add
    Product Description

    Furosemide-d5 is intended for use as an internal standard for the quantification of furosemide (Item No. 17273) by GC- or LC-MS. Furosemide is a loop diuretic and an inhibitor of the Na+/K+/2Cl- (NKCC) cotransporters, NKCC1 and NKCC2 (Kis = ~10 µM for both).1,2 In vivo, furosemide (0.1 mg/kg, p.o.) increases diuresis in beagle dogs.3 Furosemide (30 mg/kg) reduces ventricular collagen deposition and fibrosis in a rat model of dilated cardiomyopathy.4 It is also an inhibitor of carbonic anhydrase I (CAI), CAII, and CAIII (Kis = 0.052-0.065 µM) and organic ion transporter 1 (OAT1; Ki = 9.5 µM), as well as a GABAA receptor antagonist.5,6,7

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gillen, C.M., Brill, S., Payne, J.A., et al. Molecular cloning and functional expression of the K-Cl cotransporter from rabbit, rat, and human. A new member of the cation-chloride cotransporter family. The Journal of Biological Chemisty 271(27), 16237-16244 (1996).

    2. Markadieu, N., and Delpire, E. Physiology and pathophysiology of SLC12A1/2 transporters. Pflugers Arch. 466(1), 91-105 (2014).

    3. Potter, B.M., Ames, M.K., Hess, A., et al. Comparison between the effects of torsemide and furosemide on the reninangiotensin-aldosterone system of normal dogs. J. Vet. Cardiol. 26, 51-62 (2019).

    4. Watanabe, K., Sreedhar, R., Thandavarayan, R.A., et al. Comparative effects of torasemide and furosemide on gap junction proteins and cardiac fibrosis in a rat model of dilated cardiomyopathy. Biofactors 43(2), 187-194 (2017).

    5. Temperini, C., Cecchi, A., Scozzafava, A., et al. Carbonic anhydrase inhibitors. Interaction of indapamide and related diuretics with 12 mammalian isozymes and X-ray crystallographic studies for the indapamide-isozyme II adduct. Bioorg. Med. Chem. Lett. 18(8), 2567-2573 (2008).

    6. Rajan, S.T., Kumar, M.K., and Ramakrishna, M. The novel process for the preparation of (1R,2R,3aS,9aS)-[[2,3,3a,4,9,9a-hexahydro-2-hydroxy-1-[(3S)-3-hydroxyoctyl]-1H-benz[f]inden-5-yl]-oxy]acetic acid. (2014).

    7. Siess, W., Siegel, F.L., and Lapetina, E.G. Dihomogammalinolenic acid, but not eicosapentaenoic acid, activates washed human platelets. Biochim. Biophys. Acta 801(2), 265-276 (1984).