Active • Host: HEK293 cells • AA: 35-319 • Tag: C-terminal His • MW: 32.8 kDa
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

CEACAM8/CD66b (human, recombinant)

Item No. 31834

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Carcinoembryonic Antigen CGM6
  • Carcinoembryonic Antigen-related Cell Adhesion Molecule 8
  • CGM6
  • NCA-95
  • Non-specific Cross-reacting Antigen NCA-95
Purity
≥96% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg, determined by the LAL endotoxin assay
Source
Active recombinant human C-terminal His-tagged CD66b expressed in HEK293 cells
Amino Acids
35-319
MW
32.8 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
UniProt Accession №
P31997
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $361

    Add

    1 mg

    $2,345

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    CD66b, also known as carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), is a member of the carcinoembryonic antigen (CEA) family encoded by CEACAM8 in humans.1,2 It is a single-chain glycoprotein, comprised of N-terminal immunoglobulin (Ig) variable-like (IgV) and Ig constant-like (IgC) domains, that is anchored to the cell membrane by a glycosylphosphatidylinositol (GPI) linker.3 CD66b is expressed exclusively in granulocytes, with neutrophil and eosinophil expression increasing under inflammatory conditions to mediate intercellular adhesion to endothelial cells, signal transduction, and cell activation.1,3 The percentage of colonic CD66b+ neutrophils is increased and positively correlates with endoscopic disease severity in patients with Crohn’s disease.4 The percentage of CD66b+ neutrophils is also increased in gastric cancer tissues and is associated with poor prognosis.5 Cayman’s CEACAM8/CD66b (human, recombinant) protein can be used for binding assays. The protein consists of 296 amino acids, has a calculated molecular weight of 32.8 kDa, and a predicted N-terminus of Gln35 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 55 to 60 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yoon, J., Terada, A., and Kita, H. CD66b regulates adhesion and activation of human eosinophils. J. Immunol. 179(12), 8454-8462 (2007).

    2. Skubitz, K.M., Campbell, K.D., and Skubitz, A.P.N. CD66a, CD66b, CD66c, and CD66d each independently stimulate neutrophils. J. Leukoc. Biol. 60(1), 106-117 (1996).

    3. Skubitz, K.M., and Skubitz, A.P.N. Interdependency of CEACAM-1, -3, -6, and -8 induced human neutrophil adhesion to endothelial cells. J. Transl. Med. 6, 78 (2008).

    4. Therrien, A., Chapuy, L., Bsat, M., et al. Recruitment of activated neutrophils correlates with disease severity in adult Crohn’s disease. Clin. Exp. Immunol. 195(2), 251-264 (2019).

    5. Huang, X., Pan, Y., Ma, J., et al. Prognostic significance of the infiltration of CD163+ macrophages combined with CD66b+ neutrophils in gastric cancer. Cancer Med. 7(5), 1731-1741 (2018).