LIGHT/CD258 Extracellular Domain (human, recombinant)

LIGHT, also known as CD258, is a type II transmembrane glycoprotein encoded by TNFSF14 in humans.¹ Alternative splicing of the TNFSF14 pre-mRNA produces a cytosolic isoform, as well as a membrane-bound isoform, which can be proteolytically cleaved by metalloproteinases to produce a soluble form.^2,3 It exists as a homotrimer and is constitutively expressed on immature dendritic cells and transiently expressed on activated T cells.^3,1 LIGHT is also expressed by other hematopoietic cells, including natural killer (NK) cells, monocytes, and granulocytes.^4,2 It binds to herpes virus entry mediator (HVEM) (Item No. 31840), also known as CD270, as well as the lymphotoxin β receptor (LTβR), where it acts as a costimulatory molecule for T, B, and dendritic cells by promoting cytokine secretion, leading to cell differentiation and activation.^2,5 Tnfsf14^-/- mice have increased mortality in a model of experimental autoimmune encephalomyelitis (EAE), whereas Tnfsf14^-/- mice have reduced inflammation in a model of graft versus host disease (GVHD), indicating additional roles in immune regulation.^6,1 Cayman’s LIGHT/CD258 Extracellular Domain (human, recombinant) protein can be used for enzyme activity assays. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of LIGHT/CD258 (amino acids 74-240) fused to human IgG1 Fc at its N-terminus, consists of 404 amino acids, and has a calculated molecular weight of 44.9 kDa.

WARNING This product is not for human or veterinary use.

1. Ware, C.F. Targeting the LIGHT-HVEM pathway. Therapeutic Targets of the TNF Superfamily 146-155 (2009).

2. del Rio, M.-L., Lucas, C.L., Buhler, L., et al. HVEM/LIGHT/BTLA/CD160 cosignaling pathways as targets for immune regulation. J. Leukoc. Biol. 87(2), 223-235 (2009).

3. del Rio, M.-L., Fernandez-Renedo, C., Chaloin, O., et al. Immunotherapeutic targeting of LIGHT/LTβR/HVEM pathway fully recapitulates the reduced cytotoxic phenotype of LIGHT-deficient T cells. MAbs 8(3), 478-490 (2016).

4. Shui, J.-W., and Kronenberg, M. HVEM: An unusual TNF receptor family member important for mucosal innate immune responses to microbes. Gut Microbes 4(2), 146-151 (2013).

5. Shui, J.-W., Steinberg, M.W., and Kronenberg, M. Regulation of inflammation, autoimmunity, and infection immunity by HVEM-BTLA signaling. J. Leukoc. Biol. 89(4), 517-523 (2011).

6. Maña, P., Liñares, D., Silva, D.G., et al. LIGHT (TNFSF14/CD258) is a decisive factor for recovery from experimental autoimmune encephalomyelitis. J. Immunol. 191(1), 154-163 (2013).