Active • Host: HEK293 cells • AA: 2-94 • Tag: C-terminal human IgG1 Fc • MW: 37.1 kDa
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S100A1 (human, recombinant)

Item No. 31844

Product Insert (PDF)
Technical Information
Synonyms
  • S100α
  • S100 Calcium-binding Protein A1
  • S100 Protein α Chain
  • S100 Protein Subunit α
Purity
≥95% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/µg, determined by the LAL endotoxin assay
Source
Active recombinant C-terminal human IgG1 Fc-tagged S100A1 expressed in HEK293 cells
Amino Acids
2-94
MW
37.1 kDa
Formulation (Request formulation change)
Lyophilized from sterile 50 mM Tris, pH 7.5, with 100 mM glycine and 10 mM sodium chloride
UniProt Accession №
P23297
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

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    100 µg

    $361

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    Product Description

    S100 calcium-binding protein A1 (S100A1) is a calcium-binding protein that exists as a homodimer, with each subunit containing two EF-hand calcium-binding domains connected by a hinge region and a C-terminal extension.1 It is expressed primarily in ventricular cardiomyocytes but also in skeletal muscle and vascular endothelial cells.1,2 In cardiomyocytes it is localized to the sarcoplasmic reticulum, mitochondria, and myofilaments where it regulates calcium homeostasis, contractile machinery, and cellular energy metabolism. S100A1 is localized to the cytoplasm with perinuclear enrichment in micro- and macrovascular endothelial cells and regulates responses to adrenergic receptor activation, vessel relaxation, and angiogenesis.2,3 Overexpression of S100A1 is protective against the development of heart failure in post-ischemic isolated mouse cardiomyocytes and reduces mortality in a mouse model of myocardial infarction.3 S100A1 knockdown impairs post-ischemic angiogenesis and neovascularization in mice.2 S100A1 levels are inversely correlated with disease severity in patients with heart failure. Cayman’s S100A1 (human, recombinant) protein can be used for binding assay applications. This protein is a disulfide-linked homodimer. The reduced monomer, composed of S100A1 (amino acids 2-94) fused to human IgG1 Fc at its C-terminus, consists of 330 amino acids and has a calculated molecular weight of 37.1 kDa. As a result of glycosylation, the monomer migrates at 40 kDa by SDS-PAGE under reducing conditions.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Völkers, M., Rohde, D., Goodman, C., et al. S100A1: A regulator of striated muscle sarcoplasmic reticulum Ca2+ handling, sarcomeric, and mitochondrial function. J. Biomed. Biotechnol. 178614, (2010).

    2. Rohde, D., Busch, M., Volkert, A., et al. Cardiomyocytes, endothelial cells and cardiac fibroblasts: S100A1’s triple action in cardiovascular pathophysiology. Future Cardiol. 11(3), 309-321 (2015).

    3. Duarte-Costa, S., Castro-Ferreira, R., Neves, J.S., et al. S100A1: A major player in cardiovascular performance. Physiol. Res. 63(6), 669-681 (2014).