For immunochemical detection of SARS-CoV-2 spike glycoprotein S2 subunit ECD
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SARS-CoV-2 Spike Glycoprotein S2 Subunit Extracellular Domain Chimeric Monoclonal Antibody (Clone D001)

Item No. 31998

Product Insert (PDF)
Technical Information
Synonyms
  • 2019-nCoV Surface Glycoprotein S2 Subunit
  • COVID-19 Spike Glycoprotein S2 Subunit
  • SARS-CoV-2 Spike Glycoprotein S2 Subunit
  • Severe Acute Respiratory Syndrome Coronavirus 2 Spike Glycoprotein S2 Subunit
Immunogen
Recombinant C-terminal His-tagged SARS-CoV-2 spike glycoprotein S2 subunit ECD
Clone Designation
D001
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
0.2 μm filtered solution in PBS
Host
Chimeric Monoclonal Antibody
Isotype
Human IgG1
Applications
ELISA, FC, ICC, IF
Cross Reactivity
(+) SARS-CoV-2 spike glycoprotein S2 subunit(+) SARS-CoV-2 Omicron (XBB) spike glycoprotein RBD(+) SARS-CoV-2 Omicron (BQ.1.1) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 Omicron (BF.7) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 (BA.4.6) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 (BA.2.75) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 Omicron (BA.2.75.2) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 Omicron (BA.1.1) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 Omicron (B.1.1.529) S1+S2 trimer(+) SARS-CoV-2 Omicron (BA.2) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 XD (BA.1 x AY.4) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 Delta (B.1.617.2) spike glycoprotein S1+S2 trimer(+) SARS-CoV-2 Delta (B.1.617.2) spike glycoprotein S1+S2(+) SARS-CoV-2 spike glycoprotein S1+S2 ECD(+) SARS-CoV spike glycoprotein S1+S2 ECD(+) MERS-CoV spike glycoprotein S1+S2 ECD(-) SARS-CoV-2 Omicron (BQ.1.1) spike glycoprotein RBD(-) SARS-CoV-2 Omicron (BA.4.6/BF.7) spike glycoprotein RBD(-) SARS-CoV-2 (BA.2.75) spike glycoprotein RBD(-) SARS-CoV-2 Omicron (BA.2.75.2) spike glycoprotein RBD(-) SARS-CoV-2 (BA.2.3.20) spike glycoprotein RBD(-) SARS-CoV-2 Omicron (BA.1.1) spike glycoprotein RBD(-) SARS-CoV-2 Omicron (B.1.1.529) spike glycoprotein S1 subunit(-) SARS-CoV-2 Omicron (B.1.1.529) spike glycoprotein RBD(-) SARS-CoV-2 Omicron (BA.2) spike glycoprotein S1 subunit(-) SARS-CoV-2 Omicron (BA.2) spike glycoprotein RBD(-) SARS-CoV-2 Omicron (BA.2) spike glycoprotein S1 subunit NTD(-) SARS-CoV-2 Omicron (BA.2.12.1) spike glycoprotein RBD(-) SARS-CoV-2 Omicron (BA.4) spike glycoprotein RBD(-) SARS-CoV-2 Omicron (BA.5) spike glycoprotein RBD(-) SARS-CoV-2 Delta (B.1.617.2) spike glycoprotein S1 subunit(-) SARS-CoV-2 Delta (B.1.617.2) spike glycoprotein RBD(-) SARS-CoV-2 Delta (B.1.617.2) spike glycoprotein S1 subunit NTD(-) SARS-CoV-2 spike glycoprotein S1 subunit(-) SARS-CoV-2 spike glycoprotein RBD(-) HCoV-OC43 spike glycoprotein S1+S2(-) HCoV-NL63 spike glycoprotein S1+S2(-) HCoV-HKU1 (isolate N5) spike glycoprotein S1+S2(-) HCoV-229E spike glycoprotein S1+S2
Species Reactivity
(+) SARS-CoV-2(-) MERS-CoV
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    Product Description

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus, a member of the Betacoronavirus genus, and the causative agent of COVID-19.1,2,3,4,5 The SARS-CoV-2 spike glycoprotein, also known as the surface glycoprotein, is a viral structural protein encoded by the S gene in SARS-CoV-2 RNA.1 It is composed of an S1 and S2 subunit divided by a furin S-cleavage site not found in other SARS-CoVs.6,7 The C-terminal S2 subunit, which facilitates fusion between viral and host cell membranes, contains a fusion peptide (FP) and two heptad repeats (HRs), as well as transmembrane and cytoplasmic domains.7,8 Upon insertion of the FP in the target cell membrane, the HRs form a six-helical bundle (6-HB) that enables SARS-CoV-2 to fuse with the target cell. The SARS-CoV-2 spike glycoprotein S2 subunit increases amyloid-β (1-40) (Aβ40) and Aβ42 levels in primary mouse neuron culture supernatants and the number of hippocampal and cortical Aβ plaques in APPswe/PSEN1dE9 transgenic mice.9 Cayman's SARS-CoV-2 Spike Glycoprotein S2 Subunit Extracellular Domain Chimeric Monoclonal Antibody (Clone D001) is composed of human IgG1κ constant domains and variable regions from a mouse immunized with purified recombinant SARS-CoV-2 spike glycoprotein S2 subunit extracellular domain (ECD). It can be used for ELISA, flow cytometry (FC), immunocytochemistry (ICC), and immunofluorescence (IF) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Kandeel, M., Ibrahim, A., Fayez, M., et al. From SARS and MERS CoVs to SARS-CoV-2: Moving toward more biased codon usage in viral structural and nonstructural genes. J. Med. Virol. 92(6), 660-666 (2020).

    2. Lu, R., Zhao, X., Li, J., et al. Genomic characterisation and epidemiology of 2019 novel coronavirus: Implications for virus origins and receptor binding. Lancet 395(10224), 565-574 (2020).

    3. Meo, S.A., Alhowikan, A.M., Al-Khlaiwi, T., et al. Novel coronavirus 2019-nCoV: Prevalence, biological and clinical characteristics comparison with SARS-CoV and MERS-CoV. Eur. Rev. Med. Pharmacol. Sci. 24(4), 2012-2019 (2020).

    4. Klok, F.A., Kruip, M.J.H.A., van der Meer, N.J.M., et al. Incidence of thrombotic complications in critically ill ICU patients with COVID-19. Thromb. Res. 191, 145-147 (2020).

    5. Yang, F., Shi, S., Zhu, J., et al. Analysis of 92 deceased patients with COVID-19. J. Med. Virol. 92(11), 2511-2515 (2020).

    6. Liu, Z., Xiao, X., Wei, X., et al. Composition and divergence of coronavirus spike proteins and host ACE2 receptors predict potential intermediate hosts of SARS-CoV-2. J. Med. Virol. 92(6), 595-601 (2020).

    7. Walls, A.C., Park, Y.-J., Tortorici, M.A., et al. Structure, function, and antigenicity of the SARS-CoV-2 spike glycoprotein. Cell 181(2), 281-292 (2020).

    8. Huang, Y., Yang, C., Xu, X.-F., et al. Structural and functional properties of SARS-CoV-2 spike protein: Potential antivirus drug development for COVID-19. Acta Pharmacol. Sin. 41(9), 1141-1149 (2020).

    9. Ma, G., Zhang, D.-F., Zou, Q.-C., et al. SARS-CoV-2 Spike protein S2 subunit modulates γ-secretase and enhances amyloid-β production in COVID-19 neuropathy. Cell Discov. 8(1), 99 (2022).