Active • Host: HEK293 cells • AA: 23-268 • Tag: C-terminal His • MW: 27.7 kDa
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DS-TNFR2 M196R variant (human, recombinant)

Item No. 32023

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CD120b
  • TNFR2
  • DS-TNFR2(Δ7,8)
  • TNFRSF1B
  • Tumor Necrosis Factor Receptor 2
  • Tumor Necrosis Factor Receptor Superfamily Member 1B
Purity
≥95% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant human C-terminal His-tagged DS-TNFR2 M196R variant expressed in HEK293 cells
Amino Acids
23-268
MW
27.7 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $361

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    1 mg

    $2,345

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    Product Description

    TNF receptor 2 (TNFR2), also known as CD120b or TNFRSF1B, is a TNF-α receptor encoded by TNFRSF1B and expressed in cells of hematopoietic origin, as well as endothelial cells.1,2,3 Alternative splicing leads to the formation of a soluble, secreted isoform of TNFR2, known as differential splicing TNFR2 (DS-TNFR2), lacking the transmembrane and cytoplasmic domains.1 Cell supernatants containing DS-TNFR2 inhibit TNF-α-induced cytotoxicity in L929 cells. Serum levels of DS-TNFR2 are elevated in patients with rheumatoid arthritis. Single nucleotide polymorphisms in TNFRSF1B resulting in a methionine-to-arginine substitution at position 196 (M196R) are associated with susceptibility to systemic lupus erythematosus (SLE) in Japanese patients, rheumatoid arthritis risk in European patients, and polycystic ovary syndrome (PCOS) in female patients.4,3,2 Cayman's DS-TNFR2 M196R variant (human, recombinant) protein can be used for cell-based assay applications. This protein consists of 257 amino acids, has a calculated molecular weight of 27.7 kDa, and a predicted N-terminus of Leu23 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 43 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Lainez, B., Fernandez-Real, J.M., Romero, X., et al. Identification and characterization of a novel spliced variant that encodes human soluble tumor necrosis factor receptor 2. Int. Immunol. 16(1), 169-177 (2004).

    2. Peral, B., San Millán, J.L., Castello, R., et al. Comment: The methionine 196 arginine polymorphism in exon 6 of the TNF receptor 2 gene (TNFRSF1B) is associated with the polycystic ovary syndrome and hyperandrogenism. J. Clin. Endocrinol. Metab. 87(8), 3977-3983 (2002).

    3. Song, G.G., Bae, S.-C., and Lee, Y.H. Associations between functional TNFR2 196 M/R polymorphisms and susceptibility to rheumatoid arthritis: A meta-analysis. Rheumatol. Int. 34(11), 1529-1537 (2014).

    4. Komata, T., Tsuchiya, N., Matsushita, M., et al. Association of tumor necrosis factor receptor 2 (TNFR2) polymorphism with susceptibility to systemic lupus erythematosus. Tissue Antigens 53(6), 527-533 (1999).