Host: Insect cells • AA: 215-900 • Tag: C-terminal His • MW: 80 kDa
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PDE2A (human, recombinant; aa 215-900)

Item No. 32037

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CGS-PDE
  • CGMP-Dependent 3',5'-Cyclic Phosphodiesterase
  • Cyclic GMP-Stimulated Phosphodiesterase
  • Phosphodiesterase 2A, CGMP-Stimulated
Purity
≥90% estimated by SDS-PAGE
Source
Recombinant human C-terminal His-tagged PDE2A expressed in insect cells
Amino Acids
215-900
MW
80 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20mM Tris, pH 7.4, with 500 mM sodium chloride and 10% glycol
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    50 µg

    $361

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    Cayman Chemical
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    Product Description

    Phosphodiesterase 2A (PDE2A) is a cGMP-stimulated cyclic nucleotide phosphodiesterase that hydrolyzes cGMP and cAMP.1 It is a 941-amino acid protein comprised of an N-terminal domain (1-214), GAF-A domain (215-372), GAF-B domain (393-541), and a catalytic domain (579-941) and functions as a homodimer.2 PDE2A is expressed in the brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney, spleen, and pituitary.3 Upon cGMP binding to the GAF-B domain, PDE2A is activated and hydrolyzes cAMP and cGMP to regulate cell signaling.1 PDE2A mRNA levels are reduced in postmortem amygdala from patients with schizophrenia, bipolar disorder, and major depressive disorder compared with healthy controls.4 PDE2A brain activity is increased in the Fmr1-/- mouse model of fragile X syndrome and administration of the selective PDE2A inhibitor BAY 60-7550 (Item No. 10011135) restores axonal length and spine maturation and attenuates social discriminatory and vocalization deficits in Fmr1-/- young mice.5 Adenovirus shRNA knockdown of Pde2a attenuates bronchoalveolar lavage fluid (BALF) neutrophilia, reduces lung levels of inducible nitric oxide synthase (iNOS), and increases lung cAMP levels in a mouse model of acute lung injury.6 Cayman’s PDE2A (human, recombinant; aa 215-900) protein consists of 706 amino acids and has a calculated molecular weight of 80 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 66 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Zhang, C., Lueptow, L.M., Zhang, H.-T., et al. The role of phosphodiesterase-2 in psychiatric and neurodegenerative disorders. Phosphodiesterases: CNS Functions and Diseases 17, (2017).

    2. Pandit, J., Forman, M.D., Fennell, K.F., et al. Mechanism for the allosteric regulation of phosphodiesterase 2A deduced From the X-ray structure of a near full-length construct. Proc. Natl. Acad. Sci. USA 106(43), 18225-18230 (2009).

    3. Stephenson, D.T., Coskran, T.M., Wilhelms, M.B., et al. Immunohistochemical localization of phosphodiesterase 2A in multiple mammalian species. J. Histochem. Cytochem. 57(10), 933-949 (2009).

    4. Farmer, R., Burbano, S.D., Patel, N.S., et al. Phosphodiesterases PDE2A and PDE10A both change mRNA expression in the human brain with age, but only PDE2A changes in a region-specific manner with psychiatric disease. Cell. Signal. 70, 109592 (2020).

    5. Maurin, T., Melancia, F., Jarjat, M., et al. Involvement of phosphodiesterase 2A activity in the pathophysiology of fragile X syndrome. Cereb. Cortex 29(8), 3241-3252 (2019).

    6. Rentsendorj, O., Damarla, M., Aggarwal, N.R., et al. Knockdown of lung phosphodiesterase 2A attenuates alveolar inflammation and protein leak in a two-hit mouse model of acute lung injury. Am. J. Physiol. Lung Cell. Mol. Physiol. 301(2), 161-170 (2011).