Host: Insect cells • AA: 1-533 (full length) • Tag: N-terminal His-GST • MW: 89.5 kDa
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PDE9A2 (human, recombinant)

Item No. 32039

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • High Affinity cGMP-specific 3',5'-cyclic Phosphodiesterase 9A
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Recombinant human N-terminal His-GST-tagged PDE9A2 expressed in insect cells
Amino Acids
1-533 (full length)
MW
89.5 kDa
Formulation (Request formulation change)
Lyophilized from sterile 20 mM Tris, pH 7.4, with 500 mM sodium chloride, 5 mM GSH, and 10% glycerol
UniProt Accession №
O76083-2
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $422

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    Cayman Chemical
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    Product Description

    Phosphodiesterase 9A (PDE9A) is a high-affinity cGMP-specific cyclic nucleotide phosphodiesterase.1 Alternative splicing in the 5' region of PDE9A mRNA results in a variety of PDE9A isoforms, including PDE9A2, whereas the 3' region encoding the catalytic PDE domain does not vary between isoforms.2,3 PDE9A2 mRNA is expressed in colon, prostate, spleen, peripheral blood leukocytes, small intestine, thymus, testis, and ovary.2 PDE9A2 localizes to membrane ruffles, the perinuclear region, and other membrane regions when transiently overexpressed in HeLa and COS-1 cells.3 PDE9A expression is elevated in reticulocytes and neutrophils isolated from patients with sickle cell disease compared with healthy individuals.4 Cayman's PDE9A2 (human, recombinant) protein consists of 770 amino acids and has a calculated molecular weight of 89.5 kDa. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is approximately 75 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Fisher, D.A., Smith, J.F., Pillar, J.S., et al. Isolation and characterization of PDE9A, a novel human cGMP-specific phosphodiesterase. The Journal of Biological Chemisty 273(25), 15559-15564 (1998).

    2. Rentero, C., Monfort, A., and Puigdomènech, P. Identification and distribution of different mRNA variants produced by differential splicing in the human phosphodiesterase 9A gene. Biochem. Biophys. Res. Commun. 301(3), 686-692 (2003).

    3. Dorner-Ciossek, C., Kroker, K.S., and Rosenbrock, H. Role of PDE9 in cognition. Phosphodiesterases: CNS Functions and Diseases 231-254 (2017).

    4. Almeida, C.B., Traina, F., Lanaro, C., et al. High expression of the cGMP-specific phosphodiesterase, PDE9A, in sickle cell disease (SCD) and the effects of its inhibition in erythroid cells and SCD neutrophils. Br. J. Haematol. 142(5), 836-844 (2008).