Active • Host: HEK293 cells • AA: 113-261 • Tag: N-terminal human IgG1 Fc • MW: 42.8 kDa
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Soluble CD40 Ligand (human, recombinant; Fc-tagged)

Item No. 32046

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CD40L
  • CD154
  • T-B Cell-activating Molecule
  • T Cell Antigen Gp39
  • TNF-related Activation Protein
  • TNF Superfamily Member 5
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant N-terminal human IgG1 Fc-tagged CD40 ligand expressed in HEK293 cells
Amino Acids
113-261
MW
42.8 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
UniProt Accession №
P29965
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    50 µg

    $240

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    500 µg

    $1,438

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    Cayman Chemical
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    Product Description

    CD40 ligand (CD40L) is a type II transmembrane protein and member of the TNF superfamily encoded by CD40LG in humans.1 It is expressed primarily by activated T cells, as well as activated B cells and platelets and, under inflammatory conditions, can be induced in natural killer (NK) cells, mast cells, basophils, and monocytes. CD40L trimerizes to engage its receptor, CD40, on various cells, including B cells, dendritic cells, monocytes, platelets, macrophages, endothelial cells, and myoblasts, to induce cell type-specific signaling and activities. Soluble CD40L (sCD40L) is produced by proteolytic cleavage of CD40L at the cell surface and is found in the circulation.2,3 sCD40L retains its ability to trimerize and bind to CD40, indicating CD40L can act as a cytokine.2 Exogenous administration of sCD40L increases blood brain barrier (BBB) permeability and increases disease severity in a mouse model of experimental autoimmune encephalomyelitis (EAE).4 sCD40L enhances collagen-induced aggregation of isolated human platelets and exacerbates thrombus formation in wild-type, but not CD40-/-, mice.5 Serum levels of sCD40L are positively correlated with the albumin quotient, a marker of BBB breakdown, in patients with multiple sclerosis.4 Cayman’s Soluble CD40 Ligand (human, recombinant; Fc-tagged) protein can be used for ELISA. This protein is a disulfide-linked homodimer. The reduced monomer, comprised of CD40L (amino acids 113-261) fused to human IgG1 Fc at its N-terminus, consists of 386 amino acids and has a calculated molecular weight of 42.8 kDa.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Elgueta, R., Benson, M.J., de Vries, V.C., et al. Molecular mechanism and function of CD40/CD40L engagement in the immune system. Immunol. Rev. 229(1), 152-172 (2009).

    2. van Kooten, C., and Banchereau, J. CD40-CD40 Ligand. J. Leukoc. Biol. 67(1), 2-17 (2000).

    3. Doublier, S., Zennaro, C., Musante, L., et al. Soluble CD40 ligand directly alters glomerular permeability and may act as a circulating permeability factor in FSGS. PLoS One 12(11), e0188045 (2017).

    4. Masuda, H., Mori, M., Umehara, K., et al. Soluble CD40 ligand disrupts the blood-brain barrier and exacerbates inflammation in experimental autoimmune encephalomyelitis. J. Neuroimmunol. 316, 117-120 (2018).

    5. Yacoub, D., Hachem, A., Théorêt, J.-F., et al. Enhanced levels of soluble CD40 ligand exacerbate platelet aggregation and thrombus formation through a CD40-dependent tumor necrosis factor receptor-associated factor-2/Rac1/p38 mitogen-activated protein kinase signaling pathway. Arterioscler. Thromb. Vasc. Biol. 30(12), 2424-2433 (2010).