Active • Host: HEK293 cells • AA: 51-183 • Tag: N-terminal mouse IgG1 Fc • MW: 42.1 kDa
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TNFSF4 Extracellular Domain (human, recombinant)

Item No. 32065

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CD134L
  • CD252
  • Glycoprotein Gp34
  • OX40 Ligand
  • OX40L
  • TAX Transcriptionally Activated Glycoprotein 1
  • Tumor Necrosis Factor Ligand Superfamily Member 4
  • TXGP1
Purity
≥90% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant N-terminal mouse IgG1 Fc-tagged human TNFSF4 expressed in HEK293 cells
Amino Acids
51-183
MW
42.1 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
UniProt Accession №
P23510
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    100 µg

    $361

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    1 mg

    $2,345

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    Cayman Chemical
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    Product Description

    TNF ligand superfamily member 4 (TNFSF4), also known as OX40L, is a type II transmembrane protein and the cognate ligand of the T cell co-stimulatory TNF receptor TNFRSF4, also known as OX40.1,2 TNFSF4 assembles into trimers, with each monomer possessing an approximately 132-amino acid extracellular domain comprised of a TNF homology domain followed by a C-terminal extension. It is upregulated in antigen-presenting cells, including B cells, macrophages, and dendritic cells, in response to antigen presentation, and is also expressed in mast cells, activated T cells, and certain endothelial cells.2 Binding of a TNFSF4 trimer to three TNFRSF4 monomers promotes T cell survival and memory, as well as an effector T cell phenotype, enhances cell mobility, and increases the production of effector cytokines. Polymorphisms in TNFSF4 are associated with susceptibility to systemic lupus erythematosus (SLE) and systemic sclerosis.3,4 Cayman’s TNFSF4 Extracellular Domain (human, recombinant) protein can be used for ELISA. This protein is a disulfide-lined homodimer. The reduced monomer, comprised of TNFSF4 (amino acids 51-183) fused to mouse IgG1 Fc at its N-terminus, consists of 369 amino acids and has a calculated molecular weight of 42.1 kDa. As a result of glycosylation, the monomer migrates at approximately 50 kDa by SDS-PAGE under reducing conditions.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Compaan, D.M., and Hymowitz, S.G. The crystal structure of the costimulatory OX40-OX40L complex. Structure 14(8), 1321-1330 (2006).

    2. Webb, G.J., Hirschfield, G.M., and Lane, P.J.L. OX40, OX40L and autoimmunity: A comprehensive review. Clin. Rev. Allergy Immunol. 50(3), 312-332 (2016).

    3. Lee, Y.H., and Song, G.G. Associations between TNFSF4 and TRAF1-C5 gene polymorphisms and systemic lupus erythematosus: A meta-analysis. Hum. Immunol. 73(10), 1050-1054 (2012).

    4. Gourh, P., Arnett, F.C., Tan, F.K., et al. Association of TNFSF4 (OX40L) polymorphisms with susceptibility to systemic sclerosis. Ann. Rheum. Dis. 69(3), 550-555 (2010).