Active • Host: HEK293 cells • AA: 23-202 • MW: 19.7 kDa
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LIF (human, recombinant)

Item No. 32066

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Differentiation Inhibitory Factor
  • HILDA
  • Human Interleukin in DA Cells
  • Leukemia Inhibitor Factor
  • LIF Interleukin 6 Family Cytokine
  • Melanoma-derived LPL Inhibitor
  • MLPLI
Purity
≥95% estimated by SDS-PAGE
Endotoxin Testing
<1.0 EU/μg, determined by the LAL endotoxin assay
Source
Active recombinant human LIF expressed in HEK293 cells
Amino Acids
23-202
MW
19.7 kDa
Formulation (Request formulation change)
Lyophilized from sterile PBS, pH 7.4
UniProt Accession №
P15018
Shipping & Storage Information
Storage
-80°C
Shipping
Dry ice in continental US; may vary elsewhere
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    20 µg

    $174

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    100 µg

    $453

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    1 mg

    $2,503

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    Product Description

    Leukemia inhibitory factor (LIF) is a cytokine and member of the IL-6 family with roles in cell growth and differentiation, bone metabolism, and inflammation.1 Mature LIF is a 180-amino acid peptide arranged as a four-helix bundle in an up-up-down-down configuration that is synthesized as a 202-amino acid precursor which is post-translationally processed to remove a 22-amino acid signaling sequence from its N-terminus. It is ubiquitously expressed and this expression is commonly upregulated under inflammatory conditions.2 LIF binds to its receptor LIFRβ and recruits the IL-6 family common receptor gp130 to form a heterodimer and induce intracellular signaling via the JAK-STAT pathway.1,3 Exogenous administration of LIF reduces the rate of deterioration in grip strength and slows progression of the forelimb deformity in the wobbler mouse model of amyotrophic lateral sclerosis (ALS).4 Intracerebroventricular administration of a recombinant adeno-associated viral vector encoding LIF reduces food intake and body weight gain in rats.5 Knockdown of Lif induces infertility in female mice.1 Cayman’s LIF (human, recombinant) protein can be used for cell-based assay applications. This protein consists of 180 amino acids, has a calculated molecular weight of 19.7 kDa, and a predicted N-terminus of Ser23 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 35.4 kDa due to glycosylation.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Nicola, N.A., and Babon, J.J. Leukemia inhibitory factor (LIF). Cytokine Growth Factor Rev. 26(5), 533-544 (2015).

    2. Hisaka, T., Desmoulière, A., Taupin, J.-L., et al. Expression of leukemia inhibitory factor (LIF) and its receptor gp190 in human liver and in cultured human liver myofibroblasts. Cloning of new isoforms of LIF mRNA. Comp. Hepatol. 3(10), (2004).

    3. Onishi, K., and Zandstra, P.W. LIF signaling in stem cells and development. Development 142(13), 2230-2236 (2015).

    4. Kurek, J.B., Radford, A.J., Crump, D.E., et al. LIF (AM424), a promising growth factor for the treatment of ALS. J. Neurol. Sci. 160(Suppl. 1), S106-S113 (1998).

    5. Pasquin, S., Sharma, M., and Gauchat, J.-F. Cytokines of the LIF/CNTF family and metabolism. Cytokine 82, 122-124 (2016).