LAG-3 Extracellular Domain (human, recombinant)

Lymphocyte activation gene 3 (LAG-3) protein is a CD4-like protein and member of the immunoglobulin superfamily (IgSF) that binds with high affinity to MHC class II molecules.^1,2 LAG3 is expressed in activated T cells and natural killer cells, and the protein functions as an inhibitory co-receptor that modulates T cell homeostasis, proliferation, expansion, activation, and function.^3,4,2 LAG-3 is comprised of an N-terminal extracellular domain with four IgSF domains, a transmembrane domain, and a C-terminal cytoplasmic domain with a KIEELE motif required for modulation of T cell function.^1,3,2 Elevated LAG3 expression in chronic lymphocytic leukemia (CLL) cells is associated with reduced treatment-free survival in patients with CLL.⁵ Co-administration of antibodies against both LAG-3 and PD-1 reduces the number and percentage of CLL cells in blood and spleen in a CLL-splenocyte adoptive transfer mouse model.⁶ SNPs in LAG3 are associated with susceptibility to multiple sclerosis.⁷ Cayman's LAG-3 Extracellular Domain (human, recombinant) protein consists of 433 amino acids, has a calculated molecular weight of 47 kDa, and a predicted N-terminus of Val29 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 50 to 60 kDa due to glycosylation.

WARNING This product is not for human or veterinary use.

1. Baixeras, E., Huard, B., Miossec, C., et al. Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens. J. Exp. Med. 176(2), 327-337 (1992).

2. Goldberg, M.V., and Drake, C.G. LAG-3 in cancer immunotherapy. Cancer Immunology and Immunotherapy 269-278 (2011).

3. Triebel, F., Jitsukawa, S., Baixeras, E., et al. LAG-3, a novel lymphocyte activation gene closely related to CD4. J. Exp. Med. 171(5), 1393-1405 (1990).

4. Sierro, S., Romero, P., and Speiser, D.E. The CD4-like molecule LAG-3, biology and therapeutic applications. Expert. Opin. Ther. Targets 15(1), 91-101 (2011).

5. Kotaskova, J., Tichy, B., Trbusek, M., et al. High expression of lymphocyte-activation gene 3 (LAG3) in chronic lymphocytic leukemia cells is associated with unmutated immunoglobulin variable heavy chain region (IGHV) gene and reduced treatment-free survival. J. Mol. Diagn. 12(3), 328-334 (2010).

6. Wierz, M., Pierson, S., Guyonnet, L., et al. Dual PD1/LAG3 immune checkpoint blockade limits tumor development in a murine model of chronic lymphocytic leukemia. Blood 131(14), 1617-1621 (2018).

7. Zhang, Z., Duvefelt, K., Svensson, F., et al. Two genes encoding immune-regulatory molecules (LAG3 and IL7R) confer susceptibility to multiple sclerosis. Genes Immun. 6(2), 145-152 (2005).