For immunochemical detection of histone H3.3G34W
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Histone H3.3G34W Rabbit Monoclonal Antibody (Clone RM263)

Item No. 32103

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H3.3 G34W
  • H3.3G34W
Immunogen
A peptide corresponding to histone H3.3G34W mutant
Clone Designation
RM263
Formulation (Request formulation change)
100 or 400 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, IHC, WB
Cross Reactivity
(H3.3G34W) H3.3G34W(-) H3.3
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $412

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    400 µl

    $998

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs). Metazoans have three histone variants: canonical histone H3.1, DNA replication-independent histone H3.3, and the centromeric variant CENP-A.2 Histone H3.3 is incorporated into chromatin in a DNA replication-independent manner at sites of active transcription.2,3,4 Histone H3.3G34W is a point mutation expressed in giant cell tumors of bone (GCTBs) and is associated with tumor recurrence and metastasis.5 Cayman's Histone H3.3G34W Rabbit Monoclonal Antibody (Clone RM263) can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Elsaesser, S.J., Goldberg, A.D., and Allis, C.D. New functions for an old variant: No substitute for histone H3.3. Curr. Opin. Genet. Dev. 20(2), 110-117 (2010).

    3. Bjerke, L., Mackay, A., Nandhabalan, M., et al. Histone H3.3 mutations drive pediatric glioblastoma through upregulation of MYCN. Cancer Discov. 3(5), 512-519 (2013).

    4. Tagami, H., Ray-Gallett, D., Almouzni, G., et al. Histone H3.1 and H3.3 complexes mediate nucleosome assembly pathways dependent or independent of DNA synthesis. Cell 116(1), 51-61 (2004).

    5. Yamamoto, H., Iwasaki, T., Yamada, Y., et al. Diagnostic utility of histone H3.3 G34W, G34R, and G34V mutant-specific antibodies for giant cell tumors of bone. Hum. Pathol. 73, 41-50 (2018).