For immunochemical detection of Ig light chain
Technical Support & Resources

Visit our FAQ

Contact Us

Toll Free Phone (USA and Canada Only): (888) 526-5351
Direct Phone: (734) 975-3888

Request Technical Support

Technical Support Request

To streamline the process attach the appropriate questionnaire to your inquiry.

Download IHC QuestionnaireDownload WB Questionnaire

View Our Privacy Statement for details on how we use and protect your data. In addition, this site is protected by hCaptcha and its Privacy Policy and Terms of Service apply.

Ig Light Chain (human) Rabbit Monoclonal Antibody (Clone RM129)

Item No. 32111

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin Light Chain
Immunogen
Human IgG
Clone Designation
RM129
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, IHC
Cross Reactivity
(+) Human Igĸ(+) Human Igλ(-) Cynomolgus monkey IgG(-) Goat IgG(-) Mouse IgG(-) Rat IgG(-) Rhesus monkey IgG
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Recommended Products

Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $411

    Add
    Cayman Chemical
    Visit Our Cancer Resource Center
    Find Tools & Resources to Study the Hallmarks of Cancer
    • Cancer cell signaling & regulation
    • Cancer metabolism
    • Tumor microenvironment
    EXPLORE NOW
    Product Description

    Immunoglobulins are members of the glycoprotein superfamily that play a central role in the adaptive immune response.1 They are produced by B cells and later secreted by plasma cells as antibodies.2 Immunoglobulins are composed of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.3 Mammalian immunoglobulins contain either Igκ or Igλ light chains, each of which are composed of a constant and variable domain.4 The ratio of Igκ to Igλ light-chain containing antibodies varies between species, with ratios of 20:1, 2:1, and 1:20 in mice, humans, and cattle, respectively. Igκ and Igλ free light chains (FLCs) are produced during immunoglobulin synthesis, and accumulation of these FLCs is associated with various disorders, including light-chain deposition disease, multiple myeloma, rheumatoid arthritis, diabetic nephropathy, and systemic lupus erythematosus (SLE).2,5,6 Cayman's Ig Light Chain (human) Rabbit Monoclonal Antibody (Clone RM129) be used for ELISA, flow cytometry (FC), immunocytochemistry (ICC), and immunohistochemistry (IHC) applications. The antibody recognizes both the Igĸ and Igλ light chains from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Esparvarinha, M., Nickho, H., Mohammadi, H., et al. The role of free kappa and lambda light chains in the pathogenesis and treatment of inflammatory diseases. Biomed. Pharmacother. 91, 632-644 (2017).

    3. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    4. Janeway, C.A., Jr., Travers, P., Walport, M., et al. Antigen recognition by B-cell and T-cell receptors. Immunobiology: The immune system in health and disease 103-107 (2005).

    5. Jimenez-Zepeda, V.H. Light chain deposition disease: Novel biological insights and treatment advances. Int. J. Lab. Hematol. 34(4), 347-355 (2012).

    6. Sannier, A., Hanouna, G., Daugas, E., et al. IgA kappa light and heavy chain deposition disease in multiple myeloma. Br. J. Haematol. 183(1), 13 (2018).