For immunochemical detection of IgE
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IgE (human) Rabbit Monoclonal Antibody (Clone RM122)

Item No. 32118

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin E
Immunogen
Human IgE
Clone Designation
RM122
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA
Cross Reactivity
(+) Human IgE(-) Human IgA(-) Human IgD(-) Human IgG(-) Human IgM
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $411

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    Cayman Chemical
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    Product Description

    Immunoglobulin E (IgE) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in type I hypersensitivity reactions and the immune response to parasites.1,2,3 It is synthesized by B cells and later secreted by plasma cells and is the least abundant circulating immunoglobulin in human serum.1,2 IgE consists of two light chains and two heavy chains, or ε chains, that contain one variable region and four Ig-like constant domains Cε1-Cε4, but lacks the flexible hinge region seen in IgD, IgG, and IgA.3,4 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.5 IgE binds to type I Fcε receptors (FcεRIs) on the surface of mast cells, basophils, and antigen-presenting dendritic cells.2 Multivalent antigen binding to IgE on the surface of mast cells induces IgE crosslinking and mast cell degranulation to initiate type I hypersensitivity reactions, including, but not limited to, systemic anaphylaxis, wheal and flare responses, allergic rhinitis, bronchial asthma, and food allergies. Serum levels of IgE are elevated in response to parasitic infection and IgE directly binds parasites to target the parasite for eosinophil degranulation-induced destruction. Cayman's IgE (human) Rabbit Monoclonal Antibody (Clone RM122) can be used for ELISA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Winter, W.E., Hardt, N.S., and Fuhrman, S. Immunoglobulin E: Importance in parasitic infections and hypersensitivity responses. Arch. Pathol. Lab. Med. 124(9), 1382-1385 (2000).

    3. Gould, H.J., Sutton, B.J., Beavil, A.J., et al. The biology of IGE and the basis of allergic disease. Annu. Rev. Immunol. 21, 579-628 (2003).

    4. Sutton, B.J., Davies, A.M., Bax, H.J., et al. IgE antibodies: From structure to function and clinical translation. Antibodies (Basel) 8(1), 19 (2019).

    5. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).