For immunochemical detection of IgG3
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IgG3 (human) Rabbit Monoclonal Antibody (Clone RM119)

Item No. 32122

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin G3
Immunogen
Human IgG3
Source
Rabbit
Clone Designation
RM119
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, IHC
Cross Reactivity
(+) Human IgG3 heavy chain(-) Human IgG1(-) human IgG2(-) human IgG4(-) human IgM(-) human IgA(-) human IgD(-) human IgE(-) Cynomolgus monkey(-) goat(-) mouse(-) rat(-) rhesus monkey
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $411

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    Cayman Chemical
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    Product Description

    Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.2 IgG exists as four isotypes in humans, IgG1, IgG2, IgG3, and IgG4, each of which has a distinct effector function. IgG3 production is driven by bacterial- or viral-associated antigens, including HIV-1 and Staphylococcus antigens, and occurs early in the immune response following IgM class-switching.2,5 IgG3 binds to and neutralizes pathogens, as well as activates complement and opsonizes bacteria, leading to complement-dependent cytotoxicity (CDC) and ADCC, respectively. Serum IgG3 levels are increased in patients with primary biliary cirrhosis, Sjögren’s syndrome, systemic sclerosis, or systemic lupus erythematosus (SLE).6 Cayman’s IgG3 (human) Rabbit Monoclonal Antibody (Clone RM119) can be used for ELISA, immunocytochemistry (ICC), and immunohistochemistry (IHC) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Vidarsson, G., Dekkers, G., and Rispens, T. IgG subclasses and allotypes: From structure to effector functions. Front. Immunol. 5, 520 (2014).

    3. Mayumi, M., Kuritani, T., Kubagawa, H.M., et al. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J. Immunol. 130(2), 671-677 (1983).

    4. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    5. Damelang, T., Rogerson, S.J., Kent, S.J., et al. Role of IgG3 in infectious diseases. Trends Immunol. 40(3), 197-211 (2019).

    6. Zhang, H., Li, P., Wu, D., et al. Serum IgG subclasses in autoimmune diseases. Medicine (Baltimore) 94(2), e387 (2015).