For immunochemical detection of β-actin
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β-Actin (N-Term) Rabbit Monoclonal Antibody (RM112)

Item No. 32127

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Actin β
  • Actin, Cytoplasmic 1
  • β-Cytoskeletal Actin
Immunogen
Peptide from the N-terminal region of β-actin
Clone Designation
RM112
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ICC, IHC, IP, WB
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    β-Actin is a cytoskeletal protein with roles in cell division, migration, and homeostasis that is encoded by ACTB in humans.1,2 It is ubiquitously expressed, localized to the cytosol, and conserved from birds to mammals. β-Actin is enriched at the leading edge of migrating cells and Actb-/- mouse embryonic fibroblasts (MEFs) exhibit decreased migration velocity, increased apoptosis, and decreased proliferation.1 Actb knockout is early embryonic lethal in mice, indicating that β-actin is essential for embryonic development. ACTBR183W and ACTBE346K gain-of-function mutations have been found in patients with deafness, developmental mutations, and juvenile-onset dystonia and neutrophil dysfunction, respectively.3 Cayman's β-Actin (N-Term) Rabbit Monoclonal Antibody (RM112) can be used for chromatin immunoprecipitation (ChIP), immunocytochemistry (ICC), immunohistochemistry (IHC), immunoprecipitation (IP), and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bunell, T.M., Burbach, B.J., Shimizu, Y., et al. β-Actin specifically controls cell growth, migration, and the G-actin pool. Mol. Biol. Cell 22(21), 4047-4058 (2011).

    2. Vedula, P., Kurosaka, S., Leu, N.A., et al. Diverse functions of homologous actin isoforms are defined by their nucleotide, rather than their amino acid sequence. Elife 6, e31661 (2017).

    3. Hundt, N., Preller, M., Swolski, O., et al. Molecular mechanisms of disease-related human β-actin mutations p.R183W and p.E364K. FEBS J. 281(23), 5279-5291 (2014).