For immunochemical detection of H3K4Me3
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Histone H3K4Me3 Monoclonal Antibody (RM340)

Item No. 32130

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Trimethylated Histone H3 Lysine 4
Immunogen
Peptide corresponding to H3K4Me3
Clone Designation
RM340
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, multiplex-based assays
Cross Reactivity
(-) H3K4Me1(-) H3K4Me2(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) Unmodified H3K4(-) H4K20Me1(-) H4K20Me2(-) H4K20Me3
Species Reactivity
(+) Vertebrates(+) H3K4Me3
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Trimethylation of histone H3 at lysine 4 (H3K4Me3) is found in euchromatic promoter regions and is associated with active transcription.3 It inhibits several H3K9 methyltransferases and has differential effects on the activities of the KDM7 demethylases PHF8 and KDM7A, activating and inhibiting H3K9Me3 demethylation, respectively. Mislocalization of H3K4Me3 is associated with disease progression and memory deficits in patients with Alzheimer's disease.4 Cayman’s Histone H3K4Me3 Monoclonal Antibody (RM340) can be used for chromatin immunoprecipitation (ChIP), ELISA, and multiplex-based assay applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Pack, L.R., Yamamoto, K.R., and Fujimori, D.G. Opposing chromatin signals direct and regulate the activity of lysine demethylase 4C (KDM4C). The Journal of Biological Chemisty 291(12), 6060-6070 (2016).

    4. Mastroeni, D., Delvaux, E., Nolz, J., et al. Aberrant intracellular localization of H3k4me3 demonstrates an early epigenetic phenomenon in Alzheimer’s disease. Neurobiol. Aging 36(12), 3121-3129 (2015).