For immunochemical detection of H3K4Me1
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Histone H3K4Me1 Monoclonal Antibody (RM140)

Item No. 32131

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Monomethylated Histone H3 Lysine 4
Immunogen
Peptide corresponding to H3K4Me1
Clone Designation
RM140
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, ICC, multiplex-based assay, WB
Cross Reactivity
(+) H3K4Me1(-) Unmodified histone H3 (1-19)(-) H3K4Me2(-) H3K4Me3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K14Me2(-) H3K18Me1(-) H3K18Me2(-) H3K23Me1(-) H3K23Me2(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H3K56Me1(-) H3K79Me1(-) H3K79Me2(-) H3K79Me3
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Monomethylation of histone H3 at lysine 4 (H3K4Me1) is found at active and primed enhancer regions of gene promoters and H3K4Me1-containing nucleosomes are more efficiently remodeled by the chromatin-remodeling complex BAF than unmarked nucleosomes.3,4 It is also enriched at CpG sites associated with aging in stem and differentiated cells.5 Cayman's Histone H3K4Me1 Monoclonal Antibody (RM140) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Gupta, J., Kumar, S., Li, J., et al. Histone H3 lysine 4 monomethylation (H3K4me1) and H3 lysine 9 monomethylation (H3K9me1): Distribution and their association in regulating gene expression under hyperglycaemic/hyperinsulinemic conditions in 3T3 cells. Biochimie 94(12), 2656-2664 (2012).

    4. Local, A., Huang, H., Albuquerque, C.P., et al. Identification of H3K4me1-associated proteins at mammalian enhancers. Nat. Genet. 50(1), 73-82 (2018).

    5. Fernández, A.F., Bayón, G.F., Urdinguio, R.G., et al. H3K4me1 marks DNA regions hypomethylated during aging in human stem and differentiated cells. Genome Res. 25(1), 27-40 (2015).