For immunochemical detection of H3K4Me2
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Histone H3K4Me2 Monoclonal Antibody (RM135)

Item No. 32132

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Dimethylated Histone H3 Lysine 4
Immunogen
Peptide corresponding to H3K4Me2
Clone Designation
RM135
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H3K4Me2(-) Unmodified histone H3 (1-19)(-) H3K4Me1(-) H3K4Me3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K14Me2(-) H3K18Me2(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H3K79Me1(-) H3K79Me2
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Dimethylation of histone H3 at lysine 4 (H3K4Me2) is found at active and primed, but inactive, loci.3,4 It is enriched in gene promoter regions and overlaps with transcription factor binding sites. Low tumor levels of H3K4Me2 positively correlate with decreased overall and disease-free survival in pancreatic cancer patients.5 Cayman's Histone H3K4Me2 Monoclonal Antibody (RM135) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Sims, R.J., III, and Reinberg, D. Histone H3 Lys 4 methylation: Caught in a bind? Genes Dev. 20(20), 2779-2786 (2006).

    4. Wang, Y., Li, X., and Hu, H. H3K4me2 reliably defines transcription factor binding regions in different cells. Genomics 103(2-3), 222-228 (2014).

    5. Watanabe, T., Morinaga, S., Akaike, M., et al. The cellular level of histone H3 lysine 4 dimethylation correlates with response to adjuvant gemcitabine in Japanese pancreatic cancer patients treated with surgery. Eur. J. Surg. Oncol. 38(11), 1051-1057 (2012).