For immunochemical detection of H3K36Me3
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Histone H3K36Me3 Monoclonal Antibody (RM155)

Item No. 32134

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Histone H3 (Lys36me3)
  • Histone H3 (trimethyl K36)
  • Trimethyl Histone H3 (Lys36)
  • Trimethylated Histone 3 Lysine 36
Immunogen
A peptide corresponding to H3K36Me3
Clone Designation
RM155
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, Multiplex-based Assays, WB
Cross Reactivity
(+) H3K36Me3(-) Unmodified H3K4Me1(-) Unmodified H3K4Me2(-) Unmodified H3K4Me3(-) Unmodified H3K9Me1(-) Unmodified H3K9Me2(-) Unmodified H3K9Me3(-) Unmodified H3K14Me2(-) Unmodified H3K18Me1(-) Unmodified H3K18Me2(-) Unmodified H3K23Me1(-) Unmodified H3K23Me2(-) Unmodified H3K27Me1(-) Unmodified H3K27Me2(-) Unmodified H3K27Me3(-) Unmodified H3K36(-) Unmodified H3K36Me1(-) Unmodified H3K36Me2(-) Unmodified H3K56Me1(-) Unmodified H3K79Me1(-) Unmodified H3K79Me2(-) Unmodified H3K79Me3
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Trimethylation of H3K36 is increased in the G1 and early S phases of the cell cycle where it binds to and recruits the mismatch recognition protein MutSα in preparation for DNA replication and mismatch repair.3 H3K36 trimethylation is found in greater amounts on exons compared with introns in C. elegans, mouse, and human genome-wide maps of histone H3 tail methylations.4 The loss of H3K36Me3 is correlated with poor prognosis in multiple cancer types while high levels increase expression of tumor suppressor genes.5 H3K36Me3 is negatively correlated with esophageal squamous cell carcinoma (ESCC) metastasis and positively correlated with increased survival in patients with ESCC. Cayman’s Histone H3K36Me3 Monoclonal Antibody (RM155) can be used for chromatin immunoprecipitation (ChIP), ELISA, multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Li, F., Mao, G., Tong, D., et al. The histone mark H3K36me3 regulates human DNA mismatch repair through its interaction with MutSα. Cell 153(3), 590-600 (2013).

    4. Kolasinska-Zwierz, P., Down, T., Latorre, I., et al. Differential chromatin marking of introns and expressed exons by H3K36me3. Nat. Genet. 41(3), 376-381 (2009).

    5. Zhou, M., Li, Y., Lin, S., et al. H3K9me3, H3K36me3, and H4K20me3 expression correlates with patient outcome in esophageal squamous cell carcinoma as epigenetic markers. Dig. Dis. Sci. 64(8), 2147-2157 (2019).