For immunochemical detection of H3K79Me1
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Histone H3K79Me1 Monoclonal Antibody (RM147)

Item No. 32137

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Monomethylated Histone H3 Lysine 79
Immunogen
Peptide corresponding to H3K79Me1
Clone Designation
RM147
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, multiplex-based assays, WB
Cross Reactivity
(+) H3K79Me1(-) Unmodified H3K79(-) H3K79Me2(-) H3K79Me3(-) H3K4Me1(-) H3K4Me2(-) H3K4Me3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K18Me1(-) H3K18Me2(-) H3K23Me1(-) H3K23Me2(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H3K56Me1
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Methylation of lysine 79 on histone H3 is associated with active chromatin and the silencing of telomere-associated genes by silent information regulator (Sir) proteins.3 The conversion of H3K79Me1 to H3K79Me2/3 is associated with increased expression of mixed lineage leukemia (MLL) target genes, including Hoxa9.4 Cayman's Histone H3K79Me1 Monoclonal Antibody (RM147) can be used for chromatin immunoprecipitation (ChIP), ELISA, multiplex-based assays, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Bhaumik, S.R., Smith, E., and Shilatifard, A. Covalent modifications of histones during development and disease pathogenesis. Nat. Struct. Mol. Biol. 14(11), 1008-1016 (2007).

    4. Deshpande, A.J., Deshpande, A., Sinha, A.U., et al. AF10 regulates progressive H3K79 methylation and HOX gene expression in diverse AML subtypes. Cancer Cell 26(6), 896-908 (2014).