For immunochemical detection of H3K9Me1
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Histone H3K9Me1 Monoclonal Antibody (RM150)

Item No. 32145

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Monomethylated Histone H3 Lysine 9
Immunogen
Peptide corresponding to H3K9Me1
Clone Designation
RM150
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H3K9Me1(-) Unmodified histone H3 (1-19)(-) H3K9Me2(-) H3K9Me3(-) H3K4Me1(-) H3K4Me2(-) H3K4Me3(-) H3K14Me2(-) H3K18Me1(-) H3K18Me2(-) H3K23Me1(-) H3K23Me2(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H3K56Me1(-) H3K79Me1(-) H3K79Me2(-) H3K79Me3
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Monomethylation of histone H3 at lysine 9 (H3K9Me1) is found at euchromatin regions of silenced genes and is correlated with gene repression.3 Recognition of H3K9Me1 by the H3K9 demethylases GLP and G9a is essential to embryonic stem cell differentiation and viability in mice.4 Cytoplasmic localization of H3K9Me1 is associated with reduced disease-specific mortality risk in patients with oral and/or oropharyngeal squamous cell carcinoma.5 Cayman's Histone H3K9Me1 Monoclonal Antibody (RM150) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Gupta, J., Kumar, S., Li, J., et al. Histone H3 lysine 4 monomethylation (H3K4me1) and H3 lysine 9 monomethylation (H3K9me1): Distribution and their association in regulating gene expression under hyperglycaemic/hyperinsulinemic conditions in 3T3 cells. Biochimie 94(12), 2656-2664 (2012).

    4. Liu, N., Zhang, Z., Wu, H., et al. Recognition of H3K9 methylation by GLP is required for efficient establishment of H3K9 methylation, rapid target gene repression, and mouse viability. Genes Dev. 29(4), 379-393 (2015).

    5. Maia, L.L., Peterle, G.T., Dos Santos, M., et al. JMJD1A, H3K9me1, H3K9me2 and ADM expression as prognostic markers in oral and oropharyngeal squamous cell carcinoma. PLoS One 13(3), e019884 (2018).