For immunochemical detection of IgG4
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IgG4 (human) Rabbit Monoclonal Antibody - Biotinylated (RM120)

Item No. 32146

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Immunoglobulin G4
Immunogen
Peptide corresponding to the hinge region of human IgG4
Clone Designation
RM120
MW
~150 and 50 kDa for non-reduced and reduced IgG4, respectively
Formulation (Request formulation change)
50 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, IHC, WB
Cross Reactivity
(+) IgG4(-) Human IgG1(-) IgG2(-) IgG3(-) IgM(-) IgA(-) IgD(-) IgE(-) Goat IgG(-) Mouse IgG(-) Rat IgG
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    50 µg

    $411

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    Cayman Chemical
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    Product Description

    Immunoglobulin G (IgG) is a member of the immunoglobulin superfamily of glycoproteins that plays a central role in the adaptive immune response.1 It is produced by B cells and later secreted by plasma cells and is the most abundant circulating antibody in human and mouse serum.1,2,3 IgG consists of two heavy chains of approximately 50 kDa each and two light chains of approximately 25 kDa each.1 The heavy chains are linked together by disulfide bonds to form an Fc region and also combine with the light chains to form the Fab region, which mediate receptor and antigen binding, respectively.4 IgG is produced following IgM class-switching in response to infection and is involved in numerous humoral host defense responses, including antibody-dependent cell-mediated cytotoxicity (ADCC), toxin neutralization, and pathogen opsonization.2 IgG exists as four isotypes in humans, IgG1, IgG2, IgG3, and IgG4, each of which has a distinct effector function. IgG4 is the least predominant IgG isotype in human serum and contains a serine at position 228 in its hinge region that facilitates Fab arm exchange, resulting in two antigen-binding sites and a functionally monovalent antibody.5 Increased serum levels of IgG4 positively correlate with desensitization and allergen tolerance in beekeepers, laboratory workers chronically exposed to rodent allergens, and individuals undergoing allergy immunotherapy for severe allergies to cats, dust mites, birch pollen, and wasps. Cayman's IgG4 (human) Rabbit Monoclonal Antibody - Biotinylated (RM120) can be used for ELISA, immunocytochemistry (ICC), immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes the heavy chain of non-reduced and reduced IgG4 at approximately 150 and 50 kDa, respectively, from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Schroeder, H.W., Jr., and Cavicini, L. Structure and function of immunoglobulins. J. Allergy Clin. Immunol. 125(2 Suppl. 2), S41-S52 (2010).

    2. Vidarsson, G., Dekkers, G., and Rispens, T. IgG subclasses and allotypes: From structure to effector functions. Front. Immunol. 5, 520 (2014).

    3. Mayumi, M., Kuritani, T., Kubagawa, H.M., et al. IgG subclass expression by human B lymphocytes and plasma cells: B lymphocytes precommitted to IgG subclass can be preferentially induced by polyclonal mitogens with T cell help. J. Immunol. 130(2), 671-677 (1983).

    4. Vaillant, A.A.J., and Ramphul, K. Immunoglobulin. 2020, (2020).

    5. Trampert, D.C., Hubers, L.M., van de Graaf, S.F.J., et al. On the role of IgG4 in inflammatory conditions: lessons for IgG4-related disease. Biochim. Biophys. Acta Mol. Basis Dis. 1864(4 Pt. B), 1401-1409 (2018).