For immunochemical detection of H3K79Me3
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Histone H3K79Me3 Monoclonal Antibody (RM157)

Item No. 32147

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Trimethylated Histone H3 Lysine 79
Immunogen
A peptide corresponding to H3K79Me3
Clone Designation
RM157
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, multiplex-based assays, WB
Cross Reactivity
(+) H3K79Me3(-) Unmodified H3K79(-) H3K79Me1(-) H3K79Me2(-) H3K4Me1(-) H3K4Me2(-) H3K4Me3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K14Me2(-) H3K18Me1(-) H3K18Me2(-) H3K23Me1(-) H3K23Me2(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H3K56Me1
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Trimethylation of histone H3 at lysine 79 (H3K79Me3) is found at promoter regions of both active and silenced genes in human CD4+ T cells and positively correlates with transcriptional repression.3 H3K79Me3 is also found at pericentromeric heterochromatin in mouse oocytes and somatic cells.4 Levels of H3K79Me3 are decreased in human tumor tissue compared to non-cancerous tissue.5 Cayman’s Histone H3K79Me3 Monoclonal Antibody (RM157) can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Barski, A., Cuddapah, S., Cui, K., et al. High-resolution profiling of histone methylations in the human genome. Cell 129(4), 823-837 (2007).

    4. Ooga, M., Inoue, A., Kageyama, S., et al. Changes in H3K79 methylation during preimplantation development in mice. Biol. Reprod. 78(3), 413-424 (2008).

    5. Evanno, E., Godet, J., Piccirilli, N., et al. Tri-methylation of H3K79 is decreased in TGF-β1-induced epithelial-to-mesenchymal transition in lung cancer. Clin. Epigenetics 9, 80 (2017).