For immunochemical detection of H3K23Me2
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Histone H3K23Me2 Monoclonal Antibody (RM171)

Item No. 32150

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Dimethylated Histone H3 Lysine 23
Immunogen
Peptide corresponding to H3K23Me2
Clone Designation
RM171
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, multiplex-based assays, WB
Cross Reactivity
(+) H3K23Me2(-) Unmodified H3K23(-) H3K23Me1(-) H3K4Me1(-) H3K4Me3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K14Me2(-) H3K18Me1(-) H3K18Me2(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H3K56Me1(-) H3K79Me1(-) H3K79Me2(-) H3K79Me3
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Dimethylation of histone H3 at lysine 23 (H3K23Me2) is a predominant histone modification in C. elegans and is associated with heterochromatin and gene suppression.3,4 Cayman’s Histone H3K23Me2 Monoclonal Antibody (RM171) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Vandamme, J., Sidoli, S., Mariani, L., et al. H3K23me2 is a new heterochromatic mark in Caenorhabditis elegans. Nucleic Acids Res. 43(20), 9694-9710 (2015).

    4. Myers, T.R., Amendola, P.G., Lussi, Y.C., et al. JMJD-1.2 controls multiple histone post-translational modifications in germ cells and protects the genome from replication stress. Sci. Rep. 8(1), 3765 (2018).