For immunochemical detection of H3K79Me2
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Histone H3K79Me2 Monoclonal Antibody (RM181)

Item No. 32151

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Dimethylated Histone H3 Lysine 79
Immunogen
Peptide corresponding to H3K79Me2
Clone Designation
RM181
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ChIP, ELISA, IHC, multiplex-based assays, WB
Cross Reactivity
(-) H3K4Me1(-) H3K4Me2(-) H3K4Me3(-) H3K9Me1(-) H3K9Me2(-) H3K9Me3(-) H3K14Me2(-) H3K18Me1(-) H3K18Me2(-) H3K23Me1(-) H3K23Me2(-) H3K27Me1(-) H3K27Me2(-) H3K27Me3(-) H3K36Me1(-) H3K36Me2(-) H3K36Me3(-) H3K56Me1(+) H3K79Me2(-) H3K79Me1(-) H3K79Me3(-) Unmodified H3K79
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Dimethylation of histone H3 at lysine 79 (H3K79Me2) is a cell cycle-dependent modification associated with gene repression that is low during G1 and early S phases, increases during late S phase, and is maintained during G2/M.3 Levels of H3K79Me2 are increased during mitosis in a histone H2B monoubiquitination-dependent manner, and loss of H3K79Me2 is associated with increased chromosome numbers and mitotic defects in HCT116 cells.4 H3K79Me2 is completely absent in postmortem brain tissue isolated from fetuses with spina bifida.5 Cayman’s Histone H3K79Me2 Monoclonal Antibody (RM181) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Mellor, J. Linking the cell cycle to histone modifications: Dot1, G1/S, and cycling K79me2. Mol. Cell. 35(6), 729-730 (2009).

    4. Guppy, B.J., and McManus, K.J. Mitotic accumulation of dimethylated lysine 79 of histone H3 is important for maintaining genome integrity during mitosis in human cells. Genetics 199(2), 423-433 (2015).

    5. Zhang, Q., Xue, P., Li, P., et al. Histone modification mapping in human brain reveals aberrant expression of histone H3 lysine 79 dimethylation in neural tube defects. Neurobiol. Dis. 54, 404-413 (2013).