Histone H4S1Ph Monoclonal Antibody (RM194)

Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.¹ Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.^1,2 Phosphorylation of histone H4 at serine 1 (H4S1Ph) is increased during the S-phase and mitosis in HeLa cells, C. elegans, and Drosophila, as well as during spermatogenesis in mice.^3,4 MG-63 cells expressing a serine-to-alanine substitution at Ser¹ in histone H4, which abolishes its phosphorylation, have reduced markers of autophagy. Cayman's Histone H4S1Ph Monoclonal Antibody (RM194) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assays, and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

3. Barber, C.M., Turner, F.B., Wang, Y., et al. The enhancement of histone H4 and H2A serine 1 phosphorylation during mitosis and S-phase is evolutionarily conserved. Chromosoma 112(7), 360-371 (2004).

4. Krishnamoorthy, T., Chen, X., Govin, J., et al. Phosphorylation of histone H4 Ser¹ regulates sporulation in yeast and is conserved in fly and mouse spermatogenesis. Genes Dev. 20(18), 2580-2592 (2006).