For immunochemical detection of H3K4
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Histone H3K4 Monoclonal Antibody (RM186)

Item No. 32160

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Unmodified Histone H3 Lysine 4
Immunogen
Peptide corresponding to the N-terminus of histone H3
Clone Designation
RM186
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) Unmodified histone H3 (1-9)(+) H3R2Me1 (1-9)(+) H3R2Me2 (1-9)(+) H3T3Ph (1-9)(-) H3K4Ac (1-9)(-) H3K4Me1 (1-9)(-) H3K4Me2 (1-9)(-) H3K4Me3 (1-9)
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

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    Product Description

    Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.1,2 Histone H3 lysine 4 (H3K4) is subject to acetylation and mono-, di-, and trimethylation, and these modifications are found in euchromatic promoter regions and associated with active transcription.3,4 Cayman's Histone H3K4 Monoclonal Antibody (RM186) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assays, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

    3. Pack, L.R., Yamamoto, K.R., and Fujimori, D.G. Opposing chromatin signals direct and regulate the activity of lysine demethylase 4C (KDM4C). The Journal of Biological Chemisty 291(12), 6060-6070 (2016).

    4. Guillemette, B., Drogaris, P., Lin, H.-H.S., et al. H3 lysine 4 is acetylated at active gene promoters and is regulated by H3 lysine 4 methylation. PLoS Genet. 7(3), e1001354 (2011).