For immunochemical detection of H2AS1Ph
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Alternative(s)
13535Histone H2A Polyclonal Antibody
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Histone H2AS1Ph Monoclonal Antibody (RM216)

Item No. 32163

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H2ApS1
  • H2ApSer1
  • H2A (Phospho-Ser1)
  • Phospho-Histone H2A Serine 1
  • Phosphorylated Histone H2A Serine 1
Immunogen
Peptide corresponding to H2AS1Ph
Clone Designation
RM216
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H2AS1Ph(+) H4S1Ph(-) Unmodified H2AS1(-) Unmodified H4S1
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

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    Product Description

    Histone H2A is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.1,2 Phosphorylation of histone H2A at serine 1 (H2AS1Ph) is mediated by p90 ribosomal S6 kinase 5 (RSK5) and is associated with transcriptional repression.3 H2AS1Ph is enriched during mitosis in C. elegans, Drosophila, and HeLa cells, as well as during meiotic recombination in mouse spermatozoa.4,5,6 Cayman's Histone H2AS1Ph Monoclonal Antibody (RM216) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications. This antibody is cross-reactive with histone H4 phosphorylated at serine 1 (H4S1Ph).

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

    3. Zhang, Y., Griffin, K., Mondal, N., et al. Phosphorylation of histone H2A inhibits transcription on chromatin templates. The Journal of Biological Chemisty 279(21), 21866-21872 (2004).

    4. Barber, C.M., Turner, F.B., Wang, Y., et al. The enhancement of histone H4 and H2A serine 1 phosphorylation during mitosis and S-phase is evolutionarily conserved. Chromosoma 112(7), 360-371 (2004).

    5. Chioccarelli, T., Pierantoni, R., Manfrevola, F., et al. Histone post-translational modifications and circRNAs in mouse and human spermatozoa: Potential epigenetic marks to assess human sperm quality. J. Clin. Med. 9(3), 640 (2020).

    6. Zhang, Z.-H., Mu, S.-M., Guo, M.-S., et al. Dynamics of histone H2A, H4 and HS1ph during spermatogenesis with a focus on chromatin condensation and maturity of spermatozoa. Sci. Rep. 6, 25089 (2016).