Histone H3T3Ph Monoclonal Antibody (RM159)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.¹ It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.^1,2 Phosphorylation of histone H3 at threonine 3 (H3T3Ph), catalyzed by the serine/threonine protein kinase haspin, is involved in the localization of the chromosomal passenger complex (CPC) at centromeres during mitosis and chromatin condensation during meiosis.^3,4 Cayman’s Histone H3T3Ph Monoclonal Antibody (RM159) can be used for ELISA, immunocytochemistry (ICC), immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

3. Kelly, A.E., Ghenoiu, C., Xue, J.Z., et al. Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B. Science 330(6001), 235-239 (2010).

4. Wang, Q., Wei, H., Du, J., et al. H3 Thr³ phosphorylation is crucial for meiotic resumption and anaphase onset in oocyte meiosis. Cell Cycle 15(2), 213-224 (2016).