Histone H3T11Ph Monoclonal Antibody (RM164)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.¹ It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination.^1,2 Phosphorylation of histone H3 at threonine 11 (H3T11Ph) occurs during mitosis in mammalian cells and localizes to the centromeric regions of chromosomes during metaphase.³ H3T11 can be phosphorylated by multiple kinases such as PKC-related kinase (PRK1), which mediates androgen-induced phosphorylation of H3T11 at the androgen receptor target genes PSA and KLK2 in LNCaP cells, and checkpoint kinase 1 (Chk1), which phosphorylates H3T11 at the promoter regions of the genes encoding cyclin B1 and Cdk1 in mice. ^4,5 High levels of H3T11Ph in tumor tissue isolated from patients with prostate cancer correlate with high Gleason scores, which are associated with aggressive tumors.⁴ Cayman’s Histone H3T11Ph Monoclonal Antibody (RM164) can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.

WARNING This product is not for human or veterinary use.

1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

2. Sharda, A., Amnekar, R.V., Natu, A., et al. Histone posttranslational modifications: Potential role in diagnosis, prognosis, and therapeutics of cancer. Prognostic Epigenetics 15, 351-373 (2019).

3. Cerutti, H., and Casas-Mollano, J.A. Histone H3 phosphorylation: Universal code or lineage specific dialects? Epigenetics 4(2), 71-75 (2009).

4. Metzger, E., Yin, N., Wissmann, M., et al. Phosphorylation of histone H3 at threonine 11 establishes a novel chromatin mark for transcriptional regulation. Nat. Cell Biol. 10(1), 53-60 (2008).

5. Moraes, I., and Casas-Mollano, J.A. Histone H3 phosphorylation in plants and other organisms. Epigenetics in plants of agronomic importance: Fundamentals and applications 47-70 (2014).