For immunochemical detection of H4K20Me3
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Histone H4K20Me3 Monoclonal Antibody (RM208)

Item No. 32170

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Trimethylated Histone H4 Lysine 20
Immunogen
Peptide corresponding to H4K20Me3
Clone Designation
RM208
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, multiplex-based assays, WB
Cross Reactivity
(+) H4K20Me3(+) H4K20Me2 (weakly)(-) Unmodified H4K20(-) H4K20Me1(-) H4K5Me1(-) H4K12Me1
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.1 Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications, such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.1,2 Trimethylation of histone H4 at lysine 20 (H4K20Me3) is enriched at pericentric heterochromatin and is associated with gene silencing.3 In senescent cells, H4K20Me3 is enriched at senescence-associated heterochromatin foci (SAHF), and levels of H4K20Me3 are increased in senescent cells compared with proliferating cells.4 H4K20Me3 levels are elevated in tumor tissue, compared with adjacent non-tumor tissue, isolated from patients with esophageal squamous cell carcinoma, and elevated H4K20Me3 levels are associated with lymph node involvement and poor prognosis in these patients.5 Cayman's Histone H4K20Me3 Monoclonal Antibody (RM208) can be used for ELISA, multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

    2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    3. Schotta, G., Lachner, M., Sarma, K., et al. A silencing pathway to induce H3-K9 and H4-K20 trimethylation at constitutive heterochromatin. Genes Dev. 18(11), 1251-1262 (2004).

    4. Nelson, D.M., Jaber-Hijazi, F., Cole, J.J., et al. Mapping H4K20me3 onto the chromatin landscape of senescent cells indicates a function in control of cell senescence and tumor suppression through preservation of genetic and epigenetic stability. Genome Biol. 17(1), 158 (2016).

    5. Zhou, M., Li, Y., Lin, S., et al. H3K9me3, H3K36me3, and H4K20me3 expression correlates with patient outcome in esophageal squamous cell carcinoma as epigenetic markers. Dig. Dis. Sci. 64(8), 2147-2157 (2019).