For immunochemical detection of H2BS14Ph
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Histone H2BS14Ph Monoclonal Antibody (Clone RM238)

Item No. 32175

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H2B (Phospho-Ser14)
  • H2BpS14
  • H2BpSer14
  • Phospho-Histone H2B Serine 14
  • Phosphorylated Histone H2B Serine 14
Immunogen
Peptide corresponding to H2BS14Ph
Clone Designation
RM238
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H2BS14Ph(-) Other phosphorylated histones
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

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    Product Description

    Histone H2B is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.1 It is a globular protein that contains a histone fold domain with a C-terminal α-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation.1,2,3 Phosphorylation of histone H2B at serine 14 (H2BS14Ph) is associated with DNA damage and induces apoptotic chromatin condensation.4,5 H2BS14Ph also correlates with class switch recombination and somatic hypermutation in germinal center B cells.6 Cayman’s H2BS14Ph Monoclonal Antibody (Clone RM238) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    2. Wyrick, J.J., and Parra, M.A. The role of histone H2A and H2B post-translational modifications in transcription: A genomic perspective. Biochim. Biophys. Acta 1789(1), 37-44 (2009).

    3. Wang, C.-Y., Hua, C.-Y., Hsu, H.-E., et al. The C-terminus of histone H2B is involved in chromatin compaction specifically at telomeres, independently of its monoubiquitylation at lysine 123. PLoS One 6(7), e22209 (2011).

    4. Wang, C., Pi, E., Zhan, Q., et al. Involvement of histone PTMs in DNA repair processes in relation to age-associated neurodegenerative disease. DNA Repair and Human Health 615-628 (2011).

    5. Ahn, S.-H., Diaz, R.L., Grunstein, M., et al. Histone H2B deacetylation at lysine 11 is required for yeast apoptosis induced by phosphorylation of H2B at serine 10. Mol. Cell 24(2), 211-220 (2006).

    6. Odegard, V.H., Kim, S.T., Anderson, S.M., et al. Histone modifications associated with somatic hypermutation. Immunity 23(1), 101-110 (2005).