For immunochemical detection of 25-hydroxy vitamin D3
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Measurement and Detection Tool(s)
900068325-hydroxy Vitamin D3
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25-hydroxy Vitamin D3 Rabbit Monoclonal Antibody (Clone RM3)

Item No. 32179

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Calcidiol
  • Calcifediol
  • 25(OH)D3
  • 25-OH Vitamin D3
Immunogen
25-hydroxy Vitamin D3-BSA conjugate
Clone Designation
RM3
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA
Cross Reactivity
(+) 25-hydroxy Vitamin D3(+) 1α,25-hydroxy Vitamin D3(-) Vitamin D3(-) Vitamin D2(-) 25-hydroxy Vitamin D2(-) 1α,25-hydroxy Vitamin D2
Species Reactivity
(Independent) Species
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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    100 µl

    $411

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    Cayman Chemical
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    Product Description

    25-hydroxy Vitamin D3 is an intermediate in the biosynthesis of 1α,25-dihydroxy vitamin D3 (calcitriol; Item No. 71820), a vitamin D receptor (VDR) agonist that has multiple functions, including stimulation of calcium absorption, insulin secretion, and cell proliferation and differentiation.1,2,3 It is produced from vitamin D3 (Item No. 11792) by 25-hydroxylase/CYP2R1 and bound to vitamin D binding protein (DBP) and albumin in the liver, then secreted into the bloodstream where it is the major circulating form of vitamin D.1 25-hydroxy Vitamin D3 is transported to the kidney where it is hydroxylated by 1α-hydroxylase/CYP27B1 or 24-hydroxylase/CYP24A1 to produce 1α,25-dihydroxy vitamin D3 or 24,25-dihydroxy vitamin D3, respectively. Conversion of 25-hydroxy vitamin D3 to 1α,25-dihydroxy vitamin D3 is stimulated by parathyroid hormone (PTH) and inhibited by calcium, phosphorus, or FGF23. Serum 25-hydroxy vitamin D3 levels have been widely used as a marker of vitamin D status.2 Increased serum 25-hydroxy vitamin D3 levels are associated with increased bone density in patients with osteoarthritis, as well as reduced risk of colorectal, breast, and prostate cancer, whereas decreased serum 25-hydroxy vitamin D3 levels have been found in children with nutritional rickets, a condition caused by vitamin D deficiency and characterized by reduced bone density.2,4,5 Cayman's 25-hydroxy Vitamin D3 Rabbit Monoclonal Antibody (Clone RM3) can be used for ELISA.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bikle, D. Vitamin D: Production, metabolism, and mechanisms of action. Endotext (2000).

    2. Brandi, M.L., and Minisola, S. Calcidiol [25(OH)D3]: From diagnostic marker to therapeutical agent. Curr. Med. Res. Opin. 29(11), 1565-1572 (2013).

    3. Lips, P. Vitamin D physiology. Prog. Biophys. Mol. Biol. 92(1), 4-8 (2006).

    4. Bischoff-Ferrari, H.A., Zhang, Y., Kiel, D.P., et al. Positive association between serum 25-hydroxyvitamin D level and bone density in osteoarthritis. Arthritis Rheum. 53(6), 821-826 (2005).

    5. Dogan, M., Erol, M., Cesur, Y., et al. The effect of 25-hydroxyvitamin D3 on the immune system. J. Pediatr. Endocrinol. Metab. 22(10), 929-935 (2009).