Histone H2A (C-Term) Monoclonal Antibody (Clone RM225)

Histone H2A is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.¹ It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to a variety of post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation, which function as epigenetic regulators of transcription.^2,1 Histone H2A contains a C-terminal tail that, in contrast to other core histones, also extends outside of the nucleosome core and is highly variable, yielding a number of H2A variants that are subject to PTMs and differentially regulate nucleosome stability and chromatin structure.³ Serum histone H2A autoantibodies have been found in patients with drug-induced or spontaneous systemic lupus erythematosus (SLE).⁴ Cayman's Histone H2A (C-Term) Monoclonal Antibody (Clone RM225) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications. This antibody recognizes the C-terminal region of histone H2A independent of PTMs.

WARNING This product is not for human or veterinary use.

1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

2. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

3. Corujo, D., and Buschbeck, M. Post-translational modifications of H2A histone variants and their role in cancer. Cancers (Basel) 10(3), 59 (2018).

4. Burlingame, R.W., and Rubin, R.L. Autoantibody to the nucleosome subunit (H2A-H2B)-DNA is an early and ubiquitous feature of lupus-like conditions. Mol. Biol. Rep. 23(3-4), 159-166 (1996).