Histone H2B (C-Term) Monoclonal Antibody (Clone RM230)

Histone H2B is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei.¹ It is a globular protein that contains a histone fold domain with a C-terminal α-helix that facilitates nucleosome interactions and chromatin compaction, as well as an unstructured N-terminal tail that extends outside of the nucleosome core, both of which are subject to various post-translational modifications (PTMs), including ubiquitination, acetylation, methylation, and phosphorylation.^1,2,3 Histone H2B PTMs function as epigenetic regulators of gene transcription by affecting chromatin structure, influencing the interaction between transcriptional regulatory proteins with DNA, and regulating several cellular functions including gene expression, apoptosis, and DNA repair.^2,3,4,5 Increased tumor expression of HIST1H2BJ, the gene encoding wild-type histone H2B, is associated with prolonged overall survival and improved prognosis in patients with cervical cancer.⁶ Serum histone H2B autoantibodies have been found in patients with drug-induced or spontaneous systemic lupus erythematosus (SLE).⁷ Cayman's Histone H2B (C-Term) Monoclonal Antibody (Clone RM230) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications. This antibody recognizes the C-terminus of histone H2B independent of PTMs.

WARNING This product is not for human or veterinary use.

1. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

2. Wyrick, J.J., and Parra, M.A. The role of histone H2A and H2B post-translational modifications in transcription: A genomic perspective. Biochim. Biophys. Acta 1789(1), 37-44 (2009).

3. Wang, C.-Y., Hua, C.-Y., Hsu, H.-E., et al. The C-terminus of histone H2B is involved in chromatin compaction specifically at telomeres, independently of its monoubiquitylation at lysine 123. PLoS One 6(7), e22209 (2011).

4. Parra, M.A., Kerr, D., Fahy, D., et al. Deciphering the roles of the histone H2B N-terminal domain in genome-wide transcription. Mol. Cell Biol. 26(10), 3842-3852 (2006).

5. Sun, Z.-W., and Allis, C.D. Ubiquitination of histone H2B regulates H3 methylation and gene silencing in yeast. Nature 418(6893), 104-108 (2002).

6. Li, X., Tian, R., Gao, H., et al. Identification of a histone family gene signature for predicting the prognosis of cervical cancer patients. Sci. Rep. 7(1), 16495 (2017).

7. Doyle, H.A., Aswad, D.W., and Mamula, M.J. Autoimmunity to isomerized histone H2B in systemic lupus erythematosus. Autoimmunity 46(1), 6-13 (2013).