For immunochemical detection of histone H2A.Z
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Histone H2A.Z (C-Term) Monoclonal Antibody (Clone RM215)

Item No. 32184

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • H2AZ
Immunogen
Peptide from the C-terminal region of human histone H2A.Z
Clone Designation
RM215
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H2A.Z independent of PTMs(-) Other histone proteins
Species Reactivity
((+)) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

    Add
    Cayman Chemical
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    Product Description

    Histone H2A.Z is a variant of histone H2A, a nuclear protein and a component of the nucleosome core.1,2 It is a globular protein containing unstructured N- and C-terminal tails that extend outside of the nucleosome core that are subject to a variety of post-translational modifications (PTMs), including acetylation, methylation, and ubiquitination, which function as epigenetic regulators of transcription.3 Histone H2A.Z influences chromatin accessibility and nucleosome stability, regulating various cellular functions, including DNA replication and repair, mitosis, heterochromatin formation, and chromosome segregation.1,4 It is enriched at gene promoters and associated with active or repressed gene transcription in a manner dependent on species or PTMs, among other factors.5,4 Increased tumor H2A.Z levels are associated with decreased overall survival in patients with breast cancer.6 Cayman's Histone H2A.Z (C-Term) Monoclonal Antibody (Clone RM215) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications. The antibody recognizes the C-terminal region of histone H2A.Z independent of PTMs.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Bönisch, C., and Hake, S.B. Histone H2A variants in nucleosomes and chromatin: More or less stable? Nucleic Acids Res. 40(21), 10719-10741 (2012).

    2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    3. Corujo, D., and Buschbeck, M. Post-translational modifications of H2A histone variants and their role in cancer. Cancers (Basel) 10(3), 59 (2018).

    4. Monteiro, F.L., Baptista, T., Amado, F., et al. Expression and functionality of histone H2A variants in cancer. Oncotarget 5(11), 3428-3443 (2014).

    5. Marques, M., Laflamme, L., Gervais, A.L., et al. Reconciling the positive and negative roles of histone H2A.Z in gene transcription. Epigenetics 5(4), 267-272 (2010).

    6. Hua, S., Kallen, C.B., Dhar, R., et al. Genomic analysis of estrogen cascade reveals histone variant H2A.Z associated with breast cancer progression. Mol. Syst. Biol. 4(1), 188 (2008).