For immunochemical detection of histone H4
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Alternative(s)
13543Histone H4 Polyclonal Antibody
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Histone H4 (C-Term) Rabbit Monoclonal Antibody (Clone RM212)

Item No. 32186

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Immunogen
Peptide from the C-terminal region of human histone H4
Clone Designation
RM212
Formulation (Request formulation change)
100 µg of protein A-affinity purified monoclonal antibody.
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
ELISA, ICC, multiplex-based assays, WB
Cross Reactivity
(+) H4A independent of PTMs(-) Other histone proteins
Species Reactivity
(+) Vertebrates
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µg

    $500

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    Product Description

    Histone H4 is one of four core histone proteins that are involved in the organization of DNA into chromatin.1 Histones are globular proteins with unstructured N-terminal tails and are subject to a variety of post-translational modifications (PTMs), such as methylation, acetylation, phosphorylation, and citrullination, that can influence chromatin structure and regulate gene transcription.1,2 Histone H4 PTMs function as epigenetic regulators of transcription by affecting chromatin structure and enhancing transcription factor binding, as well as play a key role in the maintenance of genomic integrity.3,4 Levels of histone H4 are increased in the inferior and middle temporal gyrus in postmortem brains from patients with Alzheimer’s disease.5 Histone H4 (C-Term) Rabbit Monoclonal Antibody (Clone RM212) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications. The antibody recognizes the C-terminus of histone H4 independent of PTMs.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Wang, Y., Li, M., Stadler, S., et al. Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation. J. Cell Biol. 184(2), 205-213 (2009).

    2. Hyun, K., Jeon, J., Park, K., et al. Writing, erasing and reading histone lysine methylations. Exp. Mol. Med. 49(4), e324 (2017).

    3. Vettese-Dadey, M., Grant, P.A., Hebbes, T.R., et al. Acetylation of histone H4 plays a primary role in enhancing transcription factor binding to nucleosomal DNA in vitro. EMBO J. 15(10), 2508-2518 (1996).

    4. Jørgensen, S., Schotta, G., and Sørensen, C.S. Histone H4 Lysine 20 methylation: Key player in epigenetic regulation of genomic integrity. Nucleic Acids Res. 41(5), 2797-2806 (2013).

    5. Narayan, P.J., Lill, C., Faull, R., et al. Increased acetyl and total histone levels in post-mortem Alzheimer’s disease brain. Neurobiol. Dis. 74, 281-294 (2015).