For immunochemical detection of cytokeratin 5
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Cytokeratin 5 (C-Term; human) Rabbit Monoclonal Antibody (Clone RM226)

Item No. 32190

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • CK-5
  • Keratin-5
  • Keratin, Type II Cytoskeletal 5
  • KRT5
Immunogen
Peptide from the C-terminal region of human cytokeratin 5
Clone Designation
RM226
Formulation (Request formulation change)
100 µl or 1 ml of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Cross Reactivity
(+) Cytokeratin 5(-) Cytokeratin 6
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    Cytokeratin 5 is a type II epithelial intermediate filament protein.1 It is composed of a central rod containing four α-helical domains, which are important for self-assembly, and non-helical head and tail domains at the N- and C-termini, respectively. Cytokeratin 5 is expressed in basal keratinocytes in the epidermis and is an integral component of the epithelial cell cytoskeleton.2 It dimerizes with the type I epithelial intermediate filament protein cytokeratin 14 via heptad repeats in the central rod domain to form a network of filament bundles throughout the cytoplasm.3,1 Cytokeratin 5 interacts with β-catenin in estrogen receptor-positive breast cancer cells and promotes the reduction of β-catenin levels at the cell surface in vitro and in a patient-derived xenograft (PDX) mouse model of breast cancer.4 The expression of cytokeratin 5 in cancer cells predicts poor prognosis in estrogen receptor-positive breast cancer.4 Mutations in the tail or head domains of cytokeratin 5 induce cytoskeletal abnormalities and keratin aggregation and are associated with epidermolysis bullosa simplex (EBS) while head domain mutations are associated with Dowling-Degos disease.2,1,3 Cayman’s Cytokeratin 5 (C-Term; human) Rabbit Monoclonal Antibody (Clone RM226) can be used for immunohistochemistry (IHC) and Western blot (WB) applications.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Gu, L.-H., and Coulombe, P.A. Keratin function in skin epithelia: A broadening palette with surprising shades. Curr. Opin. Cell Biol. 19(1), 13-23 (2007).

    2. Atkinson, S.D., McGilligan, V.E., Liao, H., et al. Development of allele-specific therapeutic siRNA for keratin 5 mutations in epidermolysis bullosa simplex. J. Invest. Dermatol. 131(10), 2079-2086 (2011).

    3. Chan, Y.M., Yu, Q.C., LeBlanc-Straceski, J., et al. Mutations in the non-helical linker segment L1-2 of keratin 5 in patients with Weber-Cockayne epidermolysis bullosa simplex. J. Cell Sci. 107(Pt 4), 765-774 (1994).

    4. McGinn, O., Ward, A.V., Fettig, L.M., et al. Cytokeratin 5 alters β-catenin dynamics in breast cancer cells. Oncogene 39(12), 2478-2492 (2020).