p38α MAPK (Phospho-Thr¹⁸⁰/Tyr¹⁸²) Rabbit Monoclonal Antibody (Clone RM243)

p38 MAPK is a serine/threonine protein kinase and member of the MAPK family with roles in the regulation of immune responses and embryonic development, as well as cell differentiation, metabolism, and survival.^1,2 It exists as 4 isoforms, p38α, -β, -γ, and -δ, encoded by MAPK14, MAPK11, MAPK12, and MAPK13, respectively, in humans. p38α MAPK is ubiquitously expressed, with the highest levels of expression in the heart, skeletal muscle, and brain.^1,3 It is activated via dual phosphorylation of threonine 180 (Thr¹⁸⁰) and tyrosine 182 (Tyr¹⁸²) by the MAP2K kinases MKK3 and MKK6 in response to LPS or the production of inflammatory cytokines and induces signaling through protein kinases, transcription factors, and transcriptional regulators, among others.^1,2 Levels of activated p38α MAPK (p38α phospho-Thr¹⁸⁰/Tyr¹⁸²) are increased and positively correlated with apoptosis in DU145 and PC3 prostate cancer cells in response to cisplatin (Item No. 13119).⁴ p38α Phospho-Thr¹⁸⁰/Tyr¹⁸² levels are also increased in adult rat ventricular monocytes during stimulated ischemia.⁵ Cayman's p38α MAPK (Phospho-Thr¹⁸⁰/Tyr¹⁸²) Rabbit Monoclonal Antibody (Clone RM243) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes p38α MAPK (phospho-Thr¹⁸⁰/Tyr¹⁸²) at approximately 38 kDa from human samples.

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1. Lee, J.K., and Kim, N.-J. Recent advances in the inhibition of p38 MAPK as a potential strategy for the treatment of Alzheimer’s disease. Molecules 22(8), 1287 (2017).

2. Han, J., Wu, J., and Silke, J. An overview of mammalian p38 mitogen-activated protein kinases, central regulators of cell stress and receptor signaling. F1000Res. 9, 653 (2020).

3. Bachstetter, A.D., and Van Eldik, L.J. The p38 MAP kinase family as regulators of proinflammatory cytokine production in degenerative diseases of the CNS. Aging Dis. 1(3), 199-211 (2010).

4. Skjøth, I.H.E., and Issinger, O.-G. Profiling of signaling molecules in four different human prostate carcinoma cell lines before and after induction of apoptosis. Int. J. Oncol. 28(1), 217-229 (2006).

5. Jacquet, S., Zarrinpashneh, E., Chavey, A., et al. The relationship between p38 mitogen-activated protein kinase and AMP-activated protein kinase during myocardial ischemia. Cardiovasc. Res. 76(3), 465-472 (2007).