For immunochemical detection of E-cadherin
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E-Cadherin Rabbit Monoclonal Antibody (Clone RM244)

Item No. 32198

Product Insert (PDF)Safety Data Sheet (SDS) (PDF)
Technical Information
Synonyms
  • Cadherin-1
  • CDH1
  • L-CAM
  • Uvomorulin
Immunogen
Peptide corresponding to E-cadherin
Clone Designation
RM244
MW
~120 kDa
Formulation (Request formulation change)
100 µl of protein A-affinity purified monoclonal antibody
Storage Buffer
PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide
Host
Rabbit
Isotype
IgG
Applications
IHC, WB
Species Reactivity
(+) Human
Shipping & Storage Information
Storage
-20°C
Shipping
Wet ice in continental US; may vary elsewhere
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Certificates of Analysis & Batch Specific Data

Provide batch numbers separated by commas to download or request available product inserts, QC sheets, certificates of analysis, data packs, and GC-MS data.

    100 µl

    $411

    Add
    Cayman Chemical
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    Product Description

    E-cadherin is a calcium-dependent adhesion molecule that mediates the formation of adherens junctions and regulates epithelial cell integrity and tissue formation.1,2 It is a transmembrane glycoprotein composed of an extracellular cadherin domain that mediates cell-cell interactions, a transmembrane domain, and a cytoplasmic domain that interacts with a variety of adaptor proteins, including β-catenin, to influence cytoskeletal dynamics.3,4 E-cadherin is expressed by epithelial cells and localizes to the cell surface, where it assembles into cis-cadherin dimers that subsequently form trans-cadherin dimers with cadherins expressed on adjacent cells, resulting in cell-cell adhesion.5 E-cadherin cell surface expression is regulated by clathrin-dependent or -independent endocytosis and drives cytoskeletal rearrangements that are necessary for wound closure.6 Loss of E-cadherin is a key feature of the epithelial-to-mesenchymal transition (EMT), a process that promotes tumorigenesis and cancer metastasis.1 Cayman’s E-Cadherin Rabbit Monoclonal Antibody (Clone RM244) can be used for immunohistochemistry (IHC) and Western blot (WB) applications. The antibody recognizes E-cadherin at approximately 120 kDa from human samples.

    WARNING This product is not for human or veterinary use.

    References & Product Citations
    Product Description References

    1. Yu, W., Yang, L., Li, T., et al. Cadherin signaling in cancer: Its functions and role as a therapeutic target. Front Oncol. 9, 989 (2019).

    2. van Roy, F., and Berx, G. The cell-cell adhesion molecule E-cadherin. Cell. Mol. Life Sci. 65(23), 3756-3788 (2008).

    3. Canel, M., Serrels, A., Frame, M.C., et al. E-cadherin-integrin crosstalk in cancer invasion and metastasis. J. Cell. Sci. 126(Pt 2), 393-401 (2013).

    4. Biswas, K.H., and Zaidel-Bar, R. Early events in the assembly of E-cadherin adhesions. Exp. Cell Res. 358(1), 14-19 (2017).

    5. Campbell, H.K., Maiers, J.L., and DeMali, K.A. Interplay between tight junctions & adherens junctions. Exp. Cell Res. 358(1), 39-44 (2017).

    6. Brüser, L., and Bogdan, S. Adherens junctions on the move-membrane trafficking of E-cadherin. Cold Spring Harb. Perspect. Biol. 9(3), a029140 (2017).